Integrating Epigenetic Modulators into NanoScript for Enhanced Chondrogenesis of Stem Cells

Sahishnu Patel, Thanapat Pongkulapa, Perry T. Yin, Ganesh N. Pandian, Christopher Rathnam, Toshikazu Bando, Thangavel Vaijayanthi, Hiroshi Sugiyama, Ki Bum Lee

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


N-(4-Chloro-3-(trifluoromethyl)phenyl)-2-ethoxybenzamide (CTB) is a small molecule that functions by altering the chromatin architecture to modulate gene expression. We report a new CTB derivative with increased solubility and demonstrate CTB's functionality by conjugating it on the recently established NanoScript platform to enhance gene expression and induce stem cell differentiation. NanoScript is a nanoparticle-based artificial transcription factor that emulates the structure and function of transcription factor proteins (TFs) to effectively regulate endogenous gene expression. Modifying NanoScript with CTB will more closely replicate the TF structure and enhance CTB functionality and gene expression. To this end, we first conjugated CTB onto NanoScript and initiated a time-dependent increase in histone acetyltransferase activity. Next, because CTB is known to trigger the pathway involved in regulating Sox9, a master regulator of chondrogenic differentiation, we modifed a Sox9-specific NanoScript with CTB to enhance chondrogenic gene activity and differentiation. Because NanoScript is a tunable and robust platform, it has potential for various gene-regulating applications, such as stem cell differentiation.

Original languageEnglish (US)
Pages (from-to)4598-4601
Number of pages4
JournalJournal of the American Chemical Society
Issue number14
StatePublished - Apr 15 2015

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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