Integrative modeling of the HIV-1 ribonucleoprotein complex

David S. Goodsell, Andrew Jewett, Arthur J. Olson, Stefano Forli

Research output: Contribution to journalArticle

Abstract

A coarse-grain computational method integrates biophysical and structural data to generate models of HIV-1 genomic RNA, nucleocapsid and integrase condensed into a mature ribonucleoprotein complex. Several hypotheses for the initial structure of the genomic RNA and oligomeric state of integrase are tested. In these models, integrase interaction captures features of the relative distribution of gRNA in the immature virion and increases the size of the RNP globule, and exclusion of nucleocapsid from regions with RNA secondary structure drives an asymmetric placement of the dimerized 5’UTR at the surface of the RNP globule.

Original languageEnglish (US)
Article numbere1007150
JournalPLoS computational biology
Volume15
Issue number6
DOIs
Publication statusPublished - Jun 2019

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this