Interaction of a host protein with core complexes of bacteriophage Φ6 to control transcription

Xueying Qiao, Yang Sun, Jian Qiao, Leonard Mindich

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacteriophages of the family Cystoviridae have genomes consisting of three double-stranded RNA (dsRNA) segments, L, S, and M, packaged within a polyhedral capsid along with RNA polymerase. Transcription of genomic segment L is activated by the interaction of host protein YajQ with the capsid structure. Segment L codes for the proteins of the inner capsid, which are expressed early in infection. Green fluorescent protein (GFP) fusions with YajQ produce uniform fluorescence in uninfected cells and in cells infected with viruses not dependent on YajQ. Punctate fluorescence develops when cells are infected with YajQ-dependent viruses. It appears that the host protein binds to the infecting particles and remains with them during the entire infection period.

Original languageEnglish (US)
Pages (from-to)4821-4825
Number of pages5
JournalJournal of virology
Volume84
Issue number9
DOIs
StatePublished - May 1 2010

Fingerprint

capsid
bacteriophages
Bacteriophages
transcription (genetics)
Capsid
Cystoviridae
Fluorescence
fluorescence
Viruses
viruses
Proteins
Double-Stranded RNA
proteins
double-stranded RNA
Capsid Proteins
cells
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
Green Fluorescent Proteins
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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abstract = "Bacteriophages of the family Cystoviridae have genomes consisting of three double-stranded RNA (dsRNA) segments, L, S, and M, packaged within a polyhedral capsid along with RNA polymerase. Transcription of genomic segment L is activated by the interaction of host protein YajQ with the capsid structure. Segment L codes for the proteins of the inner capsid, which are expressed early in infection. Green fluorescent protein (GFP) fusions with YajQ produce uniform fluorescence in uninfected cells and in cells infected with viruses not dependent on YajQ. Punctate fluorescence develops when cells are infected with YajQ-dependent viruses. It appears that the host protein binds to the infecting particles and remains with them during the entire infection period.",
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Interaction of a host protein with core complexes of bacteriophage Φ6 to control transcription. / Qiao, Xueying; Sun, Yang; Qiao, Jian; Mindich, Leonard.

In: Journal of virology, Vol. 84, No. 9, 01.05.2010, p. 4821-4825.

Research output: Contribution to journalArticle

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AU - Qiao, Jian

AU - Mindich, Leonard

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