Interaction of an essential Escherichia coli GTPase, Der, with the 50S Ribosome via the KH-like domain

Jihwan Hwang, Masayori Inouye

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Der, an essential Escherichia coli tandem GTPase, has been implicated in 50S subunit biogenesis. The rrmJ gene encodes a methyltransferase that modifies the U2552 residue of 23S rRNA, and its deletion causes a severe growth defect. Peculiarly, overexpression of Der suppresses growth impairment. In this study, using an rrmJ-deletion strain, we demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. We also identified a region of Der that is critical for its specific interaction with 50S subunit.

Original languageEnglish (US)
Pages (from-to)2277-2283
Number of pages7
JournalJournal of bacteriology
Volume192
Issue number8
DOIs
StatePublished - Apr 2010

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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