Interactive effects of melatonin and cytokinin on alleviating drought-induced leaf senescence in creeping bentgrass (Agrostis stolonifera)

Xiqing Ma, Jing Zhang, Patrick Burgess, Stephanie Rossi, Bingru Huang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Drought stress induces leaf senescence while cytokinins (CK) and melatonin (ME) play roles in regulating leaf senescence. The objectives of this study were to determine whether ME alone or interacting with CK could suppress drought-induced leaf senescence through mediating CK pathways and chlorophyll catabolism in creeping bentgrass (Agrostis stolonifera). Plants of wild type (‘WT’) (cv. ‘Penncross’) or a transgenic line ('s41′) harbouring isopentenyltransferase (ipt) gene were foliar sprayed with either 20 μM ME or water and exposed to drought stress for 14 d in growth chambers. Exogenous applied ME increased visual quality, photochemical efficiency, chlorophyll content and relative water content, while it suppressed leaf electrolyte leakage, lipid peroxidation and hydrogen peroxide production in both WT and ipt-transgenic plants. Cytokinin-signaling and synthesis genes were also significantly up-regulated in ME-treated ipt-transgenic plants. Dehydration responsive genes (JUB1 and DREB2A) were up-regulated and chlorophyll-degradation genes (Chlase, PPH and Chl-PRX) and enzyme activities were significantly down-regulated by ME application. Endogenous melatonin content and ME-biosynthesis genes (TDC1, SNAT1, and COMT) expression increased in ipt-transgenic plants with ME treatment. ME-alleviation of drought-induced leaf senescence in creeping bentgrass was associated with the down-regulation of chlorophyll catabolism and the synergistically interaction with CK-synthesis gene and signaling pathways.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalEnvironmental and Experimental Botany
Volume145
DOIs
StatePublished - Jan 1 2018

Fingerprint

Agrostis stolonifera
melatonin
senescence
cytokinins
drought
transgenic plant
gene
chlorophyll
leaves
catabolism
drought stress
transgenic plants
genes
dehydration
water stress
electrolyte
hydrogen peroxide
enzyme activity
leakage
effect

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Plant Science

Keywords

  • Chlorophyll degradation
  • Drought
  • Gene expression
  • Grass
  • Hormone
  • Leaf senescence

Cite this

@article{5b49346b964e4ec49affb63baf882a15,
title = "Interactive effects of melatonin and cytokinin on alleviating drought-induced leaf senescence in creeping bentgrass (Agrostis stolonifera)",
abstract = "Drought stress induces leaf senescence while cytokinins (CK) and melatonin (ME) play roles in regulating leaf senescence. The objectives of this study were to determine whether ME alone or interacting with CK could suppress drought-induced leaf senescence through mediating CK pathways and chlorophyll catabolism in creeping bentgrass (Agrostis stolonifera). Plants of wild type (‘WT’) (cv. ‘Penncross’) or a transgenic line ('s41′) harbouring isopentenyltransferase (ipt) gene were foliar sprayed with either 20 μM ME or water and exposed to drought stress for 14 d in growth chambers. Exogenous applied ME increased visual quality, photochemical efficiency, chlorophyll content and relative water content, while it suppressed leaf electrolyte leakage, lipid peroxidation and hydrogen peroxide production in both WT and ipt-transgenic plants. Cytokinin-signaling and synthesis genes were also significantly up-regulated in ME-treated ipt-transgenic plants. Dehydration responsive genes (JUB1 and DREB2A) were up-regulated and chlorophyll-degradation genes (Chlase, PPH and Chl-PRX) and enzyme activities were significantly down-regulated by ME application. Endogenous melatonin content and ME-biosynthesis genes (TDC1, SNAT1, and COMT) expression increased in ipt-transgenic plants with ME treatment. ME-alleviation of drought-induced leaf senescence in creeping bentgrass was associated with the down-regulation of chlorophyll catabolism and the synergistically interaction with CK-synthesis gene and signaling pathways.",
keywords = "Chlorophyll degradation, Drought, Gene expression, Grass, Hormone, Leaf senescence",
author = "Xiqing Ma and Jing Zhang and Patrick Burgess and Stephanie Rossi and Bingru Huang",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.envexpbot.2017.10.010",
language = "English (US)",
volume = "145",
pages = "1--11",
journal = "Environmental and Experimental Botany",
issn = "0098-8472",
publisher = "Elsevier",

}

Interactive effects of melatonin and cytokinin on alleviating drought-induced leaf senescence in creeping bentgrass (Agrostis stolonifera). / Ma, Xiqing; Zhang, Jing; Burgess, Patrick; Rossi, Stephanie; Huang, Bingru.

In: Environmental and Experimental Botany, Vol. 145, 01.01.2018, p. 1-11.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Interactive effects of melatonin and cytokinin on alleviating drought-induced leaf senescence in creeping bentgrass (Agrostis stolonifera)

AU - Ma, Xiqing

AU - Zhang, Jing

AU - Burgess, Patrick

AU - Rossi, Stephanie

AU - Huang, Bingru

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Drought stress induces leaf senescence while cytokinins (CK) and melatonin (ME) play roles in regulating leaf senescence. The objectives of this study were to determine whether ME alone or interacting with CK could suppress drought-induced leaf senescence through mediating CK pathways and chlorophyll catabolism in creeping bentgrass (Agrostis stolonifera). Plants of wild type (‘WT’) (cv. ‘Penncross’) or a transgenic line ('s41′) harbouring isopentenyltransferase (ipt) gene were foliar sprayed with either 20 μM ME or water and exposed to drought stress for 14 d in growth chambers. Exogenous applied ME increased visual quality, photochemical efficiency, chlorophyll content and relative water content, while it suppressed leaf electrolyte leakage, lipid peroxidation and hydrogen peroxide production in both WT and ipt-transgenic plants. Cytokinin-signaling and synthesis genes were also significantly up-regulated in ME-treated ipt-transgenic plants. Dehydration responsive genes (JUB1 and DREB2A) were up-regulated and chlorophyll-degradation genes (Chlase, PPH and Chl-PRX) and enzyme activities were significantly down-regulated by ME application. Endogenous melatonin content and ME-biosynthesis genes (TDC1, SNAT1, and COMT) expression increased in ipt-transgenic plants with ME treatment. ME-alleviation of drought-induced leaf senescence in creeping bentgrass was associated with the down-regulation of chlorophyll catabolism and the synergistically interaction with CK-synthesis gene and signaling pathways.

AB - Drought stress induces leaf senescence while cytokinins (CK) and melatonin (ME) play roles in regulating leaf senescence. The objectives of this study were to determine whether ME alone or interacting with CK could suppress drought-induced leaf senescence through mediating CK pathways and chlorophyll catabolism in creeping bentgrass (Agrostis stolonifera). Plants of wild type (‘WT’) (cv. ‘Penncross’) or a transgenic line ('s41′) harbouring isopentenyltransferase (ipt) gene were foliar sprayed with either 20 μM ME or water and exposed to drought stress for 14 d in growth chambers. Exogenous applied ME increased visual quality, photochemical efficiency, chlorophyll content and relative water content, while it suppressed leaf electrolyte leakage, lipid peroxidation and hydrogen peroxide production in both WT and ipt-transgenic plants. Cytokinin-signaling and synthesis genes were also significantly up-regulated in ME-treated ipt-transgenic plants. Dehydration responsive genes (JUB1 and DREB2A) were up-regulated and chlorophyll-degradation genes (Chlase, PPH and Chl-PRX) and enzyme activities were significantly down-regulated by ME application. Endogenous melatonin content and ME-biosynthesis genes (TDC1, SNAT1, and COMT) expression increased in ipt-transgenic plants with ME treatment. ME-alleviation of drought-induced leaf senescence in creeping bentgrass was associated with the down-regulation of chlorophyll catabolism and the synergistically interaction with CK-synthesis gene and signaling pathways.

KW - Chlorophyll degradation

KW - Drought

KW - Gene expression

KW - Grass

KW - Hormone

KW - Leaf senescence

UR - http://www.scopus.com/inward/record.url?scp=85032874603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032874603&partnerID=8YFLogxK

U2 - 10.1016/j.envexpbot.2017.10.010

DO - 10.1016/j.envexpbot.2017.10.010

M3 - Article

AN - SCOPUS:85032874603

VL - 145

SP - 1

EP - 11

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

ER -