Interference of cellular ferric ions with DNA extraction and the application to methods of DNA determination

Nancy E.L. Hall, David E. Axelrod

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Iron present in biological samples prevents the extraction of DNA by hot acid hydrolysis as used in both the Schneider and Ogur-Rosen procedures [Schneider, W. C. (1945) J. Biol. Chem. 161, 293-303; Ogur, M., and Rosen, G. (1950) Arch. Biochem. 25, 262-276.] for DNA determination. Purified DNA, which is normally hot acid hydrolyzable, is rendered nonhydrolyzable by the presence of trace (0.1 mm) quantities of iron (III). Addition of a metal ion chelator, such as EDTA, to samples before hot acid treatment of DNA overcomes the inhibitory effect of iron, and, since iron is a normal cellular component, it is recommended as a general procedure for DNA determination.

Original languageEnglish (US)
Pages (from-to)425-430
Number of pages6
JournalAnalytical Biochemistry
Volume79
Issue number1-2
DOIs
StatePublished - May 1 1977

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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