TY - JOUR
T1 - Interference of cellular ferric ions with DNA extraction and the application to methods of DNA determination
AU - Hall, Nancy E.L.
AU - Axelrod, David E.
N1 - Funding Information:
This work was supported by grants from NSF and Rutgers-The State University. N.E.L.H. was supported by a Genetics Training Grant from NIH. The authors would like to thank K. Squibb for atomic absorption spectroscopy measurements and J. Valentine and M. Sanders for many helpful discussions.
PY - 1977/5/1
Y1 - 1977/5/1
N2 - Iron present in biological samples prevents the extraction of DNA by hot acid hydrolysis as used in both the Schneider and Ogur-Rosen procedures [Schneider, W. C. (1945) J. Biol. Chem. 161, 293-303; Ogur, M., and Rosen, G. (1950) Arch. Biochem. 25, 262-276.] for DNA determination. Purified DNA, which is normally hot acid hydrolyzable, is rendered nonhydrolyzable by the presence of trace (0.1 mm) quantities of iron (III). Addition of a metal ion chelator, such as EDTA, to samples before hot acid treatment of DNA overcomes the inhibitory effect of iron, and, since iron is a normal cellular component, it is recommended as a general procedure for DNA determination.
AB - Iron present in biological samples prevents the extraction of DNA by hot acid hydrolysis as used in both the Schneider and Ogur-Rosen procedures [Schneider, W. C. (1945) J. Biol. Chem. 161, 293-303; Ogur, M., and Rosen, G. (1950) Arch. Biochem. 25, 262-276.] for DNA determination. Purified DNA, which is normally hot acid hydrolyzable, is rendered nonhydrolyzable by the presence of trace (0.1 mm) quantities of iron (III). Addition of a metal ion chelator, such as EDTA, to samples before hot acid treatment of DNA overcomes the inhibitory effect of iron, and, since iron is a normal cellular component, it is recommended as a general procedure for DNA determination.
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U2 - 10.1016/0003-2697(77)90417-1
DO - 10.1016/0003-2697(77)90417-1
M3 - Article
C2 - 405886
AN - SCOPUS:0017370387
VL - 79
SP - 425
EP - 430
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1-2
ER -