Interleukin-1 response to arterial antigen, lipopolysaccharide, and oxidized low density lipoprotein in ischemic heart disease

Kenneth Grasing, Vincent Rifici, Mungal Patel, Amrish Patel, Reginald Dennis, John B. Kostis

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Background: Monocytes responding to oxidized low density lipoprotein (LDL) or other antigens may initiate atherogenesis through production of interleukin-1 (IL-1) and additional cytokines. Interleukin-1 is chemotactic for circulating leukocytes, can stimulate growth of fibroblasts or smooth muscle cells, and causes activation of T- and B-lymphocytes. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 42 patients with angiographically verified ischemic heart disease (IHD) and 35 age-matched control subjects without a history of cardiac disease. Rates of proliferation and production of IL-1β were measured after peripheral blood mononuclear cells were cultured for 7 days in the presence of mitogens, arterial antigen, lipopolysaccharide, or native and oxidized forms of LDL. Results: In patients with IHD, proliferation in response to arterial antigen was either diminished or unchanged from control values. Peripheral blood mononuclear cells from IHD and control patients had similar levels of proliferation after treatment with different mitogens. Levels of IL-1β, produced after stimulation with arterial antigen or lipopolysaccharide, also did not differ for PBMCs obtained from control and IHD patients. For patients with either a stable angina pat-tern or no history of cardiac disease, PBMC cultured in the presence of native and oxidized forms of LDL released similar amounts of IL-1β. In contrast, PBMCs from 4 patients with unstable angina had increased levels of IL-1β after culture in the presence of oxidized LDL (group means ± standard deviation of 1.63 ± 1.08 pg/mL for 17 control patients, 0.% ± 0.23 pg/mL for 4 cases with stable angina, and 4.02 ± 5.91 pg/mL for 19 cases with unstable angina). These values reflect a greater than 5-fold increase in variability for IL-1β produced on exposure to oxidized LDL for patients with unstable angina relative to control patients. Conclusions: Effects of in vitro stimulation with mitogens or lipopolysaccharide are similar for PBMC obtained from normal or IHD patients. The response to arterial antigen is also not increased in cells from patients with IHD. However, PBMCs obtained from a subset of patients with unstable angina produce greater levels of IL-1β after treat-ment with oxidized, but not native, LDL.

Original languageEnglish (US)
Pages (from-to)474-482
Number of pages9
JournalJournal of Investigative Medicine
Issue number8
StatePublished - 1997

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)


  • Arterial antigen
  • Interleukin-1
  • Ischemic heart disease
  • Peripheral blood mononuclear cells
  • Proliferation


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