Interleukin-6 family cytokines: Signaling and effects in human airway smooth muscle cells

Thomas Lahiri, Johanne D. Laporte, Paul E. Moore, Reynold A. Panettieri, Stephanie A. Shore

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Interleukin (IL)-1β induces cyclooxygenase (COX)-2 expression and prostanoid formation in cultured human airway smooth muscle (HASM) cells. In other cell types, IL-6 family cytokines induce COX-2 or augment IL-1β-induced COX-2 expression. The purpose of this study was to determine whether IL-6 family cytokines were involved in COX-2 expression in HASM cells. RT-PCR was used to demonstrate that the necessary receptor components for IL-6-type cytokine binding are expressed in HASM cells. IL-6 and oncostatin M (OSM) each caused a dose-dependent phosphorylation of signal transducer and activator of transcription-3, whereas IL-11 did not. IL-6, IL-11, and OSM alone had no effect on COX-2 expression. However, OSM caused dose-dependent augmentation of COX-2 expression and prostaglandin (PG) E2 release induced by IL-1β. In contrast, IL-6 and IL-11 did not alter IL-1β-induced COX-2 expression. IL-6 did increase IL-1β-induced PGE2 formation in unstimulated cells but not in cells stimulated with arachidonic acid (AA; 10-5 M), suggesting that IL-6 effects were mediated at the level of AA release. Our results indicate that IL-6 and OSM are capable of inducing signaling in HASM cells. In addition, OSM and IL-1β synergistically cause COX-2 expression and PGE2 release.

Original languageEnglish (US)
Pages (from-to)L1225-L1232
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume280
Issue number6 24-6
DOIs
StatePublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Keywords

  • Cyclooxygenase
  • Oncostatin
  • Prostaglandin
  • Signal transducer and activator of transcription

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