Interplay between the β′ Clamp and the β′ Jaw Domains during DNA Opening by the Bacterial RNA Polymerase at σ54-dependent Promoters

Siva R. Wigneshweraraj, Dhruti Savalia, Konstantin Severinov, Martin Buck

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The bacterial RNA polymerase (RNAP) is a multi-subunit, structurally flexible, complex molecular machine, in which activities associated with DNA opening for transcription-competent open promoter complex (OC) formation reside in the catalytic β and β′ subunits and the dissociable σ subunit. OC formation is a multi-step process that involves several structurally conserved mobile modules of β, β′, and σ. Here, we present evidence that two flexible modules of β′, the β′ jaw and the β′ clamp and a conserved regulatory Region I domain of σ54, jointly contribute to the maintenance of stable DNA strand separation around the trancription start site in OCs formed at σ54-dependent promoters. Clearly, regulated interplay between the mobile modules of the β′ and the σ subunits of the RNAP appears to be necessary for stable OC formation.

Original languageEnglish (US)
Pages (from-to)1182-1195
Number of pages14
JournalJournal of molecular biology
Volume359
Issue number5
DOIs
StatePublished - Jun 23 2006

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • DNA opening
  • RNA polymerase
  • T7 gene protein (gp2)
  • promoter stability
  • sigma factors

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