Intestinal perfusion induces rapid activation of immediate-early genes in weaning rats

Lan Jiang, Heather Lawsky, Relicardo M. Coloso, Mary A. Dudley, Ronaldo P. Ferraris

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

C-fos and c-jun are immediate-early genes (IEGs) that are rapidly expressed after a variety of stimuli. Products of these genes subsequently bind to DNA regulatory elements of target genes to modulate their transcription. In rat small intestine, IEG mRNA expression increases dramatically after refeeding following a 48-h fast. We used an in vivo intestinal perfusion model to test the hypothesis that metabolism of absorbed nutrients stimulates the expression of IEGs. Compared with those of unperfused intestines, IEG mRNA levels increased up to 11 times after intestinal perfusion for 0.3-4 h with Ringer solutions containing high (100mM) fructose (HF), glucose (HG), or mannitol (HM). Abundance of mRNA returned to preperfusion levels after 8 h. Levels of c-fos and c-jun mRNA and proteins were modest and evenly distributed among enterocytes lining the villi of unperfused intestines. HF and HM perfusion markedly enhanced IEG mRNA expression along the entire villus axis. The perfusion-induced increase in IEG expression was inhibited by actinomycin-D. Luminal perfusion induces transient but dramatic increases in c-fos and c-jun expression in villus enterocytes. Induction does not require metabolizable or absorbable nutrients but may involve de novo gene transcription in cells along the villus.

Original languageEnglish (US)
Pages (from-to)R1274-R1282
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number4 50-4
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Keywords

  • Metabolism
  • Sugars
  • Transport
  • Villus

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