Is mRNA transcribed from the strand complementary to it in a DNA duplex?

WE have tested the assumption that the messenger RNA sequence is uniquely determined by the DNA sequence complementary to it. Because RNA is usually transcribed from a DNA duplex, there is the possibility that the DNA strand that is not complementary to the mRNA influences the RNA base selection. This possibility exists in models that postulate like-with-like base pairs ^1-3, triple helix models of DNA-DNA-RNA⁴, triple base interactions such as those described in tRNA⁵, and models in which RNA polymerase may confer a special type of specificity (non-Watson-Crick) to RNA base-DNA duplex interactions, as well as in experiments in which separated are compared with non-separated strands^6-7.