Isoform switching from SM-B to SM-A myosin results in decreased contractility and altered expression of thin filament regulatory proteins

Gopal Babu, Gail J. Pyne, Yingbi Zhou, Chris Okwuchukuasanya, Joseph E. Brayden, George Osol, Richard J. Paul, Robert B. Low, Muthu Periasamy

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We previously generated an isoform-specific gene knockout mouse in which SM-B myosin is permanently replaced by SM-A myosin. In this study, we examined the effects of SM-B myosin loss on the contractile properties of vascular smooth muscle, specifically peripheral mesenteric vessels and aorta. The absence of SM-B myosin leads to decreased velocity of shortening and increased isometric force generation in mesenteric vessels. Surprisingly, the same changes occur in aorta, which contains little or no SM-B myosin in wild-type animals. Calponin and activated mitogen-activated protein kinase expression is increased and caldesmon expression is decreased in aorta, as well as in bladder. Light chain-17b isoform (LC17b) expression is increased in aorta. These results suggest that the presence or absence of SM-B myosin is a critical determinant of smooth muscle contraction and that its loss leads to additional changes in thin filament regulatory proteins.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume287
Issue number3 56-3
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

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Nonmuscle Myosin Type IIB
Myosins
Protein Isoforms
Aorta
Proteins
Muscle
Calmodulin-Binding Proteins
Gene Knockout Techniques
Wild Animals
Muscle Contraction
Mitogen-Activated Protein Kinases
Vascular Smooth Muscle
Knockout Mice
Smooth Muscle
Animals
Urinary Bladder
Genes
Light

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Babu, Gopal ; Pyne, Gail J. ; Zhou, Yingbi ; Okwuchukuasanya, Chris ; Brayden, Joseph E. ; Osol, George ; Paul, Richard J. ; Low, Robert B. ; Periasamy, Muthu. / Isoform switching from SM-B to SM-A myosin results in decreased contractility and altered expression of thin filament regulatory proteins. In: American Journal of Physiology - Cell Physiology. 2004 ; Vol. 287, No. 3 56-3.
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Isoform switching from SM-B to SM-A myosin results in decreased contractility and altered expression of thin filament regulatory proteins. / Babu, Gopal; Pyne, Gail J.; Zhou, Yingbi; Okwuchukuasanya, Chris; Brayden, Joseph E.; Osol, George; Paul, Richard J.; Low, Robert B.; Periasamy, Muthu.

In: American Journal of Physiology - Cell Physiology, Vol. 287, No. 3 56-3, 01.09.2004.

Research output: Contribution to journalArticle

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AU - Babu, Gopal

AU - Pyne, Gail J.

AU - Zhou, Yingbi

AU - Okwuchukuasanya, Chris

AU - Brayden, Joseph E.

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AU - Paul, Richard J.

AU - Low, Robert B.

AU - Periasamy, Muthu

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