Isogeneic and allogeneic lymphocyte interactions may be controlled by cell surface immunoglobulin tropism

James H. Finke, Nicholas Ponzio, J. R. Battisto

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A study of allogeneic MLC (mixed lymphocyte culture) and two types of isogeneic MLC has been conducted in which subsets of B-cells were serologically removed from pools prior to using the remainder as stimulator cells. Cellular division in the two types of ILC (isogeneic lymphocyte culture) was found to be triggered by lymphocytes with IgG1 on their surfaces. In contrast, the stimulator cells in ALC (allogeneic lymphocyte culture) possessed membrane-bound IgG2A and/or possibly IgG2B. Splenic T-cells were incapable of stimulating replication of splenic or lymph nodal T-cells in the absence of B-cells. Splenic T-cell preparations served as weak stimulators of other allogeneic T-cells but only when B-cells, either isogeneic or allogeneic to the stimulator T-cells, were present. We propose that stimulation in the MLC occurs in two distinct steps. First, immunoglobulins on cell surfaces may function to bring appropriate subsets of cells together. Next, antigenic recognition occurs that results in blastogenesis. Furthermore, the tropism or attraction that certain immunoglobulins have for some cell types may determine which subsets of cells participate in allogeneic and which take part in isogeneic MLC.

Original languageEnglish (US)
Pages (from-to)284-294
Number of pages11
JournalCellular Immunology
Volume26
Issue number2
DOIs
StatePublished - Jan 1 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology

Fingerprint Dive into the research topics of 'Isogeneic and allogeneic lymphocyte interactions may be controlled by cell surface immunoglobulin tropism'. Together they form a unique fingerprint.

Cite this