Isolation and analysis of mutants of double-stranded-RNA bacteriophage φ6 with altered packaging specificity

Jian Qiao, Xueying Qiao, Yang Sun, Leonard Mindich

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The genomes of bacteriophage φ6 and its relatives are packaged through a mechanism that involves the recognition and translocation of the three different plus strand transcripts of the segmented double-stranded RNA genomes into preformed polyhedral structures called procapsids or inner cores. This packaging requires hydrolysis of nucleoside triphosphates and takes place in the order S-M-L. Packaging is dependent on unique sequences of about 200 nucleotides near the 5′ ends of plus strand transcripts of the three genomic segments. Changes in the pac sequences lead to loss of packaging ability but can be suppressed by second-site changes in RNA or amino acid changes in protein P1, the major structural protein of the procapsid. It appears that P1 is the determinant of the RNA binding sites, and it is suggested that the binding sites overlap or are conformational changes of the same domains.

Original languageEnglish (US)
Pages (from-to)4572-4577
Number of pages6
JournalJournal of bacteriology
Volume185
Issue number15
DOIs
StatePublished - Aug 2003

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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