Isolation and analysis of the soybean SGA2 gene (cDNA), encoding a new member of the plant G-protein family of signal transducers

Cecilia Gotor, Eric Lam, Francisco J. Cejudo, Luis C. Romero

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have isolated a cDNA clone from Glycine max, named SGA2, coding for a Gα-subunit protein. The encoded polypeptide, SGα2, shows a molecular mass of 45 kDa and contains most of the conserved regions involved in guanine nucleotide binding and hydrolysis. Comparison at the nucleotide and amino acid sequence levels with the other plant Gα's shows a high degree of conservation (>85% similarity). Phylogenetic analysis of these plant genes with the other Gα's from different species clearly indicate that those proteins represent a new member of the heterotrimeric G-protein family, named Gp. Tissue localization of SGA2 transcripts in root, stem and leaf organs shows that this gene is widely expressed throughout the plant although it is most abundant in the vascular tissues of all these organs. Furthermore, the transcript is more abundant in young tissues and organ primordia than mature tissues. The high degree of sequence conservation among the plant Gα's and the differences to other species of other kingdoms, suggest that plant G proteins may function in specialized signalling processes.

Original languageEnglish (US)
Pages (from-to)1227-1234
Number of pages8
JournalPlant Molecular Biology
Volume32
Issue number6
DOIs
StatePublished - Jan 1 1996

Fingerprint

Plant Proteins
Transducers
G-proteins
Soybeans
GTP-Binding Proteins
Complementary DNA
soybeans
Genes
genes
Heterotrimeric GTP-Binding Proteins
Plant Genes
conservation plants
Guanine Nucleotides
Protein Subunits
vascular tissues
protein subunits
Blood Vessels
Glycine max
Amino Acid Sequence
polypeptides

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

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abstract = "We have isolated a cDNA clone from Glycine max, named SGA2, coding for a Gα-subunit protein. The encoded polypeptide, SGα2, shows a molecular mass of 45 kDa and contains most of the conserved regions involved in guanine nucleotide binding and hydrolysis. Comparison at the nucleotide and amino acid sequence levels with the other plant Gα's shows a high degree of conservation (>85{\%} similarity). Phylogenetic analysis of these plant genes with the other Gα's from different species clearly indicate that those proteins represent a new member of the heterotrimeric G-protein family, named Gp. Tissue localization of SGA2 transcripts in root, stem and leaf organs shows that this gene is widely expressed throughout the plant although it is most abundant in the vascular tissues of all these organs. Furthermore, the transcript is more abundant in young tissues and organ primordia than mature tissues. The high degree of sequence conservation among the plant Gα's and the differences to other species of other kingdoms, suggest that plant G proteins may function in specialized signalling processes.",
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Isolation and analysis of the soybean SGA2 gene (cDNA), encoding a new member of the plant G-protein family of signal transducers. / Gotor, Cecilia; Lam, Eric; Cejudo, Francisco J.; Romero, Luis C.

In: Plant Molecular Biology, Vol. 32, No. 6, 01.01.1996, p. 1227-1234.

Research output: Contribution to journalArticle

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