Isolation and characterization of a photoprotein, "phialidin", and a spectrally unique green-fluorescent protein from the bioluminescent jellyfish Phialidium gregarium

Leonard D. Levine, William W. Ward

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

1. 1. A calcium-activated photoprotein, "phialidin" and a green-fluorescent protein (P-GFP) have been isolated from the bioluminescent hydrozoan jellyfish, Phialidium gregarium and purified 170-fold and 500-fold, respectively. 2. 2. Phialidin has a mol wt of 23,000 ± 4% by gel filtration and a pH profile for calcium-stimulated bioluminescence between 6 and 9.5. It is less sensitive than aequorin, however, to low levels of calcium. 3. 3. With regard to mol wt (57,000 ± 4%) and thermal stability (TM = 69°C), Phialidium GFP more closely resembles Renilla GFP than Aequorea GFP. 4. 4. The corrected fluorescence emission spectrum of P-GFP resembles those of Renilla and Aequorea GFP in shape; but, having a peak emission at 497 nm, it is unique among all known GFP's.

Original languageEnglish (US)
Pages (from-to)77-85
Number of pages9
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume72
Issue number1
DOIs
StatePublished - 1982

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

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