1. 1. A calcium-activated photoprotein, "phialidin" and a green-fluorescent protein (P-GFP) have been isolated from the bioluminescent hydrozoan jellyfish, Phialidium gregarium and purified 170-fold and 500-fold, respectively. 2. 2. Phialidin has a mol wt of 23,000 ± 4% by gel filtration and a pH profile for calcium-stimulated bioluminescence between 6 and 9.5. It is less sensitive than aequorin, however, to low levels of calcium. 3. 3. With regard to mol wt (57,000 ± 4%) and thermal stability (TM = 69°C), Phialidium GFP more closely resembles Renilla GFP than Aequorea GFP. 4. 4. The corrected fluorescence emission spectrum of P-GFP resembles those of Renilla and Aequorea GFP in shape; but, having a peak emission at 497 nm, it is unique among all known GFP's.
|Original language||English (US)|
|Number of pages||9|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|State||Published - 1982|
All Science Journal Classification (ASJC) codes
- Molecular Biology