Isolation and Characterization of a Sulfated Glyceroglucolipid from Alveolar Lavage of Rabbit

Amalia SLOMIANY; Fred B. SMITH; Bronislaw L. SLOMIANY

doi:10.1111/j.1432-1033.1979.tb13158.x

Isolation and Characterization of a Sulfated Glyceroglucolipid from Alveolar Lavage of Rabbit

Amalia SLOMIANY
, Fred B. SMITH
, Bronislaw L. SLOMIANY

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE‐Sephadex and silicic acid, and thin‐layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono‐ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO₃H‐6Glcα1→6Glcαt1→6Glcα1→6Glcα1→3‐1,(3)‐O‐alkyl‐2‐O‐acylglycerol.

Original language	English (US)
Pages (from-to)	47-51
Number of pages	5
Journal	European Journal of Biochemistry
Volume	98
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-1033.1979.tb13158.x
State	Published - Jul 1979
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry

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10.1111/j.1432-1033.1979.tb13158.x

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title = "Isolation and Characterization of a Sulfated Glyceroglucolipid from Alveolar Lavage of Rabbit",

abstract = "A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE‐Sephadex and silicic acid, and thin‐layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono‐ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO3H‐6Glcα1→6Glcαt1→6Glcα1→6Glcα1→3‐1,(3)‐O‐alkyl‐2‐O‐acylglycerol.",

author = "Amalia SLOMIANY and SMITH, \{Fred B.\} and SLOMIANY, \{Bronislaw L.\}",

year = "1979",

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AU - SLOMIANY, Amalia

AU - SMITH, Fred B.

AU - SLOMIANY, Bronislaw L.

PY - 1979/7

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N2 - A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE‐Sephadex and silicic acid, and thin‐layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono‐ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO3H‐6Glcα1→6Glcαt1→6Glcα1→6Glcα1→3‐1,(3)‐O‐alkyl‐2‐O‐acylglycerol.

AB - A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE‐Sephadex and silicic acid, and thin‐layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono‐ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO3H‐6Glcα1→6Glcαt1→6Glcα1→6Glcα1→3‐1,(3)‐O‐alkyl‐2‐O‐acylglycerol.

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Isolation and Characterization of a Sulfated Glyceroglucolipid from Alveolar Lavage of Rabbit

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