Isolation and characterization of oligosaccharides from rat colonic mucus glycoprotein

B. L. Slomiany, V. L.N. Murty, A. Slomiany

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72 Scopus citations


The structure of carbohydrate units of rat colonic mucus glycoproteins was investigated. Alkaline borohydride reductive cleavage of the glycoprotein resulted in the release of a series of reduced oligosaccharides. Eight of the oligosaccharides, ranging in length from 2 to 12 sugar units, have been purified to homogeneity and their structures determined. Based on the results of immunological assays, analysis of sugar composition, sequential degradation with specific glycosidases, and methylation analyses of the intact desialyzed oligosaccharides, we propose structures for these compounds.

Original languageEnglish (US)
Pages (from-to)9719-9723
Number of pages5
JournalJournal of Biological Chemistry
Issue number20
StatePublished - 1980
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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