Isolation of lipid droplets from cells by density gradient centrifugation

Dawn L. Brasaemle; Nathan E. Wolins

doi:10.1002/cpcb.10

Isolation of lipid droplets from cells by density gradient centrifugation

Dawn L. Brasaemle, Nathan E. Wolins

School of Environmental and Biological Sciences, Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.

Original language	English (US)
Pages (from-to)	3.15.1-3.15.13.
Journal	Current Protocols in Cell Biology
Volume	2016
DOIs	https://doi.org/10.1002/cpcb.10
State	Published - 2016

All Science Journal Classification (ASJC) codes

Cell Biology

Keywords

Cell cultures
Density-gradient centrifugation
Isolation of lipid droplets
Mammalian
Preparation of lipid droplets by centrifugation

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10.1002/cpcb.10

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title = "Isolation of lipid droplets from cells by density gradient centrifugation",

abstract = "Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.",

keywords = "Cell cultures, Density-gradient centrifugation, Isolation of lipid droplets, Mammalian, Preparation of lipid droplets by centrifugation",

author = "Brasaemle, {Dawn L.} and Wolins, {Nathan E.}",

note = "Funding Information: D.B. was supported by NIH grant NIH R01 DK054797 and N.W. was supported by NIH grant NIH R01 DK088206. Publisher Copyright: {\textcopyright} 2016 by John Wiley & Sons, Inc.",

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AU - Brasaemle, Dawn L.

AU - Wolins, Nathan E.

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AB - Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.

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KW - Isolation of lipid droplets

KW - Mammalian

KW - Preparation of lipid droplets by centrifugation

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JO - Current Protocols in Cell Biology

JF - Current Protocols in Cell Biology

ER -

Isolation of lipid droplets from cells by density gradient centrifugation

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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