Isolation of yeast histone genes H2A and H2B

Lynna Hereford; Karen Fahrner; John Woolford; Michael Rosbash; David Kaback

doi:10.1016/0092-8674(79)90237-X

Isolation of yeast histone genes H2A and H2B

Lynna Hereford, Karen Fahrner, John Woolford, Michael Rosbash, David Kaback

NJMS-Molecular Core

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

Analysis of cloned sequences for yeast histone genes H2A and H2B reveals that there are only two copies of this pair of genes within the haploid yeast genome. Within each copy, the genes for H2A and H2B are separated by approximately 700 bp of spacer DNA. The two copies are separated from one another in the yeast genome by a minimum distance of 35-60 kb. Sequence homology between the two copies is restricted to the genes for H2A and H2B; the spacer DNA between the genes is nonhomologous. In both copies, the genes for H2A and H2B are divergently transcribed. In addition, both plasmids code for other nonhistone proteins. Sequences coding for histories H3 and H4 have not been detected in the immediate vicinity of the genes for H2A and H2B.

Original language	English (US)
Pages (from-to)	1261-1271
Number of pages	11
Journal	Cell
Volume	18
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(79)90237-X
State	Published - Dec 1979

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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@article{8876e20b4aad47c28acf3b3aeaac9044,

title = "Isolation of yeast histone genes H2A and H2B",

abstract = "Analysis of cloned sequences for yeast histone genes H2A and H2B reveals that there are only two copies of this pair of genes within the haploid yeast genome. Within each copy, the genes for H2A and H2B are separated by approximately 700 bp of spacer DNA. The two copies are separated from one another in the yeast genome by a minimum distance of 35-60 kb. Sequence homology between the two copies is restricted to the genes for H2A and H2B; the spacer DNA between the genes is nonhomologous. In both copies, the genes for H2A and H2B are divergently transcribed. In addition, both plasmids code for other nonhistone proteins. Sequences coding for histories H3 and H4 have not been detected in the immediate vicinity of the genes for H2A and H2B.",

author = "Lynna Hereford and Karen Fahrner and John Woolford and Michael Rosbash and David Kaback",

note = "Funding Information: We would like to thank Dr. Norman Davidson for his advice and encouragement and Lynn Golden for helpful discussions. This work was supported by three National Institute of General Medical Sciences Public Health Service grants. L.H. was also supported by a New England Medical Foundation Fellowship. D.K. and J.W. were sup- ported by NIH Postdoctoral Fellowships. M.R. is a recipient of an NIH Research Career Development Award. This is contribution No. 6091 from the Department of Chemistry, California Institute of Technology.",

year = "1979",

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doi = "10.1016/0092-8674(79)90237-X",

language = "English (US)",

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journal = "Cell",

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T1 - Isolation of yeast histone genes H2A and H2B

AU - Hereford, Lynna

AU - Fahrner, Karen

AU - Woolford, John

AU - Rosbash, Michael

AU - Kaback, David

N1 - Funding Information: We would like to thank Dr. Norman Davidson for his advice and encouragement and Lynn Golden for helpful discussions. This work was supported by three National Institute of General Medical Sciences Public Health Service grants. L.H. was also supported by a New England Medical Foundation Fellowship. D.K. and J.W. were sup- ported by NIH Postdoctoral Fellowships. M.R. is a recipient of an NIH Research Career Development Award. This is contribution No. 6091 from the Department of Chemistry, California Institute of Technology.

PY - 1979/12

Y1 - 1979/12

N2 - Analysis of cloned sequences for yeast histone genes H2A and H2B reveals that there are only two copies of this pair of genes within the haploid yeast genome. Within each copy, the genes for H2A and H2B are separated by approximately 700 bp of spacer DNA. The two copies are separated from one another in the yeast genome by a minimum distance of 35-60 kb. Sequence homology between the two copies is restricted to the genes for H2A and H2B; the spacer DNA between the genes is nonhomologous. In both copies, the genes for H2A and H2B are divergently transcribed. In addition, both plasmids code for other nonhistone proteins. Sequences coding for histories H3 and H4 have not been detected in the immediate vicinity of the genes for H2A and H2B.

AB - Analysis of cloned sequences for yeast histone genes H2A and H2B reveals that there are only two copies of this pair of genes within the haploid yeast genome. Within each copy, the genes for H2A and H2B are separated by approximately 700 bp of spacer DNA. The two copies are separated from one another in the yeast genome by a minimum distance of 35-60 kb. Sequence homology between the two copies is restricted to the genes for H2A and H2B; the spacer DNA between the genes is nonhomologous. In both copies, the genes for H2A and H2B are divergently transcribed. In addition, both plasmids code for other nonhistone proteins. Sequences coding for histories H3 and H4 have not been detected in the immediate vicinity of the genes for H2A and H2B.

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