Isoniazid resistance of exponentially growing Mycobacterium smegmatis biofilm culture

Raymond Teng; Thomas Dick

doi:10.1016/S0378-1097(03)00584-6

Isoniazid resistance of exponentially growing Mycobacterium smegmatis biofilm culture

Raymond Teng, Thomas Dick

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Biofilm growth of Mycobacterium smegmatis was found to be unaffected at an isoniazid concentration that inhibited growth of planktonic bacilli (i.e. at isoniazid minimum inhibitory concentration=10 μg ml^-1). Significant growth (50% of drug-free control) of biofilms was observed at up to 40 μg ml^-1 and the MIC for biofilm growth showed an increase to up to 80 μg ml^-1 isoniazid. Thus, the biofilm growth modus appears to be a strategy for replicating bacilli to evade the onslaught of antibacterials.

Original language	English (US)
Pages (from-to)	171-174
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	227
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(03)00584-6
State	Published - Oct 24 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

Keywords

Biofilm
Isoniazid
Mycobacterium
Phenotypic drug resistance

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10.1016/S0378-1097(03)00584-6

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title = "Isoniazid resistance of exponentially growing Mycobacterium smegmatis biofilm culture",

abstract = "Biofilm growth of Mycobacterium smegmatis was found to be unaffected at an isoniazid concentration that inhibited growth of planktonic bacilli (i.e. at isoniazid minimum inhibitory concentration=10 μg ml-1). Significant growth (50% of drug-free control) of biofilms was observed at up to 40 μg ml-1 and the MIC for biofilm growth showed an increase to up to 80 μg ml-1 isoniazid. Thus, the biofilm growth modus appears to be a strategy for replicating bacilli to evade the onslaught of antibacterials.",

keywords = "Biofilm, Isoniazid, Mycobacterium, Phenotypic drug resistance",

author = "Raymond Teng and Thomas Dick",

note = "Funding Information: We thank Pamela Thayalan for her help with some of the experiments and Calvin Boon and Indrajit Sinha for comments on the manuscript. T.D. is an adjunct staff member of the Department of Microbiology, National University of Singapore. This work was supported by the Agency for Science, Technology and Research (A*STAR), Singapore.",

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doi = "10.1016/S0378-1097(03)00584-6",

language = "English (US)",

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T1 - Isoniazid resistance of exponentially growing Mycobacterium smegmatis biofilm culture

AU - Teng, Raymond

AU - Dick, Thomas

N1 - Funding Information: We thank Pamela Thayalan for her help with some of the experiments and Calvin Boon and Indrajit Sinha for comments on the manuscript. T.D. is an adjunct staff member of the Department of Microbiology, National University of Singapore. This work was supported by the Agency for Science, Technology and Research (A*STAR), Singapore.

PY - 2003/10/24

Y1 - 2003/10/24

N2 - Biofilm growth of Mycobacterium smegmatis was found to be unaffected at an isoniazid concentration that inhibited growth of planktonic bacilli (i.e. at isoniazid minimum inhibitory concentration=10 μg ml-1). Significant growth (50% of drug-free control) of biofilms was observed at up to 40 μg ml-1 and the MIC for biofilm growth showed an increase to up to 80 μg ml-1 isoniazid. Thus, the biofilm growth modus appears to be a strategy for replicating bacilli to evade the onslaught of antibacterials.

AB - Biofilm growth of Mycobacterium smegmatis was found to be unaffected at an isoniazid concentration that inhibited growth of planktonic bacilli (i.e. at isoniazid minimum inhibitory concentration=10 μg ml-1). Significant growth (50% of drug-free control) of biofilms was observed at up to 40 μg ml-1 and the MIC for biofilm growth showed an increase to up to 80 μg ml-1 isoniazid. Thus, the biofilm growth modus appears to be a strategy for replicating bacilli to evade the onslaught of antibacterials.

KW - Biofilm

KW - Isoniazid

KW - Mycobacterium

KW - Phenotypic drug resistance

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Isoniazid resistance of exponentially growing Mycobacterium smegmatis biofilm culture

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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