Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn2+

Shuming Zhang; Hong Gu; Haoyuan Chen; Emily Strong; Edward W. Ollie; Daniel Kellerman; Danni Liang; Masaru Miyagi; Vernon E. Anderson; Joseph A. Piccirilli; Darrin M. York; Michael E. Harris

doi:10.1039/c5cc10212j

Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn²⁺

Shuming Zhang, Hong Gu, Haoyuan Chen, Emily Strong, Edward W. Ollie, Daniel Kellerman, Danni Liang, Masaru Miyagi, Vernon E. Anderson, Joseph A. Piccirilli, Darrin M. York, Michael E. Harris

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Solvent D₂O and ¹⁸O kinetic isotope effects on RNA 2′-O-transphosphorylation catalyzed by Zn²⁺ demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.

Original language	English (US)
Pages (from-to)	4462-4465
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	24
DOIs	https://doi.org/10.1039/c5cc10212j
State	Published - 2016

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c5cc10212j

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Zhang, S., Gu, H., Chen, H., Strong, E., Ollie, E. W., Kellerman, D., Liang, D., Miyagi, M., Anderson, V. E., Piccirilli, J. A., York, D. M., & Harris, M. E. (2016). Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn²⁺. Chemical Communications, 52(24), 4462-4465. https://doi.org/10.1039/c5cc10212j

Zhang, Shuming ; Gu, Hong ; Chen, Haoyuan ; Strong, Emily ; Ollie, Edward W. ; Kellerman, Daniel ; Liang, Danni ; Miyagi, Masaru ; Anderson, Vernon E. ; Piccirilli, Joseph A. ; York, Darrin M. ; Harris, Michael E. / Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn²⁺. In: Chemical Communications. 2016 ; Vol. 52, No. 24. pp. 4462-4465.

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title = "Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn2+",

abstract = "Solvent D2O and 18O kinetic isotope effects on RNA 2′-O-transphosphorylation catalyzed by Zn2+ demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.",

author = "Shuming Zhang and Hong Gu and Haoyuan Chen and Emily Strong and Ollie, {Edward W.} and Daniel Kellerman and Danni Liang and Masaru Miyagi and Anderson, {Vernon E.} and Piccirilli, {Joseph A.} and York, {Darrin M.} and Harris, {Michael E.}",

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year = "2016",

doi = "10.1039/c5cc10212j",

language = "English (US)",

volume = "52",

pages = "4462--4465",

journal = "Chemical Communications",

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Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn²⁺. / Zhang, Shuming; Gu, Hong; Chen, Haoyuan; Strong, Emily; Ollie, Edward W.; Kellerman, Daniel; Liang, Danni; Miyagi, Masaru; Anderson, Vernon E.; Piccirilli, Joseph A.; York, Darrin M.; Harris, Michael E.

In: Chemical Communications, Vol. 52, No. 24, 2016, p. 4462-4465.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn2+

AU - Zhang, Shuming

AU - Gu, Hong

AU - Chen, Haoyuan

AU - Strong, Emily

AU - Ollie, Edward W.

AU - Kellerman, Daniel

AU - Liang, Danni

AU - Miyagi, Masaru

AU - Anderson, Vernon E.

AU - Piccirilli, Joseph A.

AU - York, Darrin M.

AU - Harris, Michael E.

PY - 2016

Y1 - 2016

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AB - Solvent D2O and 18O kinetic isotope effects on RNA 2′-O-transphosphorylation catalyzed by Zn2+ demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.

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DO - 10.1039/c5cc10212j

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JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 24

ER -

Isotope effect analyses provide evidence for an altered transition state for RNA 2′-O-transphosphorylation catalyzed by Zn²⁺

Abstract

All Science Journal Classification (ASJC) codes

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