Issue engineering approach to ligament reconstruction

Michael G. Dunn, J. B. Liesch, M. L. Tiku, S. H. Maxian, J. P. Zawadsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations


Previous studies in our laboratory showed that a cellular collagen scaffold implants induce tissue ingrowth and perform similar to autografts following reconstruction of rabbit Achilles tendon of anterior cruciate ligament (ACL). We chronologically review these and related studies, and report preliminary development of fibroblast-seeded collagen scaffolds potentially useful for ACL reconstruction. The `heading potential' of fibroblast was measured within collagen scaffolds in vitro, as a function of fibroblast source. Aligned collagen scaffolds were seeded with fibroblasts from rabbit ACL, synovium, patellar tendon, or skin. Fibroblast viability, adherence, spreading, proliferation, and protein and collagen deposition were measured on collagen scaffolds. The fibroblasts attached to the scaffolds, and spread along the long axis of the collagen fibers. ACL fibroblast types however, the ACL fibroblast proliferated at the slowest rate. Patellar tendon fibroblasts proliferated at the most rapid rate. All four of the fibroblast types secreted protein and collagen within the collagen scaffolds. Preliminary in vivo studies suggest that fibroblast seeded onto collagen scaffolds can remain viable following reimplementation into the donor rabbit. Ongoing studies will elucidate the role of autogenous seeded fibroblast in neoligament formation/remodeling. These ligament analogs are potentially useful for clinical ACL reconstruction: fibroblast would be obtained from biopsy, cultured, seeded onto a collagen scaffold, and implanted as an ACL substitute into the same patient.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium Proceedings
PublisherPubl by Materials Research Society
Number of pages6
ISBN (Print)1558992308
StatePublished - 1994
EventProceedings of the Biomaterials for Drug and Cell Delivery - Boston, MA, USA
Duration: Nov 29 1993Dec 1 1993

Publication series

NameMaterials Research Society Symposium Proceedings
ISSN (Print)0272-9172


OtherProceedings of the Biomaterials for Drug and Cell Delivery
CityBoston, MA, USA

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering


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