JAK/STAT and the GATA factor Pannier control hemocyte maturation and differentiation in Drosophila

Svetlana Minakhina, William Tan, Ruth Steward

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The lymph gland is the major site of hematopoiesis in Drosophila. During late larval stages three types of hemocytes are produced, plasmatocytes, crystal cells, and lamellocytes, and their differentiation is tightly controlled by conserved factors and signaling pathways. JAK/STAT is one of these pathways which have essential roles in vertebrate and fly hematopoiesis. We show that Stat has opposing cell-autonomous and non-autonomous functions in hemocyte differentiation. Using a clonal approach we established that loss of Stat in a set of prohemocytes in the cortical zone induces plasmatocyte maturation in adjacent hemocytes. Hemocytes lacking Stat fail to differentiate into plasmatocytes, indicating that Stat positively and cell-autonomously controls plasmatocyte differentiation. We also identified the GATA factor pannier (pnr) as a downstream target of Stat. By analyzing the phenotypes resulting from clonal loss and over-expression of pnr in lymph glands, we find that Pnr is positively regulated by Stat and specifically required for the differentiation of plasmatocytes. Stat and Pnr represent two essential factors controlling blood cell maturation in the developing lymph gland and exert their functions both in a cell-autonomous and non-cell-autonomous manner.

Original languageEnglish (US)
Pages (from-to)308-316
Number of pages9
JournalDevelopmental Biology
Volume352
Issue number2
DOIs
StatePublished - Jan 1 2011

Fingerprint

GATA Transcription Factors
Hemocytes
Drosophila
Lymph
Hematopoiesis
Diptera
Vertebrates
Blood Cells
Phenotype

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Keywords

  • Drosophila
  • GATA factor
  • Hematopoiesis
  • JAK/STAT
  • Pnr

Cite this

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JAK/STAT and the GATA factor Pannier control hemocyte maturation and differentiation in Drosophila. / Minakhina, Svetlana; Tan, William; Steward, Ruth.

In: Developmental Biology, Vol. 352, No. 2, 01.01.2011, p. 308-316.

Research output: Contribution to journalArticle

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