Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker

Qin Zhou, Monique Cadrin, Harald Herrmann, Che Hong Chen, Robert J. Chalkley, Alma L. Burlingame, Bishr B. Omary

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Keratin polypeptide 20 (K20) is an intermediate filament protein with preferential expression in epithelia of the stomach, intestine, uterus, and bladder and in Merkel cells of the skin. K20 expression is used as amarker to distinguish metastatic tumor origin, but nothing is known regarding its regulation and function. We studied K20 phosphorylation as a first step toward understanding its physiologic role. K20 phosphorylation occurs preferentially on serine, with a high stoichiometry as compared with keratin polypeptides 18 and 19. Mass spectrometry analysis predicted that either K20 Ser13 or Ser14 was a likely phosphorylation site, and Ser13 was confirmed as the phospho-moiety using mutation and transfection analysis and generation of an anti-K20-phospho-Ser13 antibody. K20 Ser 13 phosphorylation increases after protein kinase C activation, and Ser13-to-Ala mutation interferes with keratin filament reorganization in transfected cells. In physiological contexts, K20 degradation and associated Ser13 hyperphosphorylation occur during apoptosis, and chemically induced mouse colitis also promotes Ser13 phosphorylation. Among mouse small intestinal enterocytes, K20 Ser13 is preferentially phosphorylated in goblet cells and undergoes dramatic hyperphosphorylation after starvation and mucin secretion. Therefore, K20 Ser13 is a highly dynamic protein kinase C-related phosphorylation site that is induced during apoptosis and tissue injury. K20 Ser13 phosphorylation also serves as a unique marker of small intestinal goblet cells.

Original languageEnglish (US)
Pages (from-to)16453-16461
Number of pages9
JournalJournal of Biological Chemistry
Issue number24
StatePublished - Jun 16 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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