A method is presented for analyzing turbidity-time curves characterizing biological assembly and disassembly processes. The method allows for the determination of apparent rate constants for the turbidity lag and growth phases of collagen fibrillogenesis. Plots of concentration/lag time versus concentration for type I collagen and concentration/(time to complete growth phase) versus concentration are shown to be straight lines. The slopes of these plots are defined as the apparent rate constants for the lag and growth phases. Activation energies are obtained from the temperature dependence of these slopes. The slopes of the growth portion of turbidity-time curves as well as the final turbidity are directly proportional to the collagen concentration. These observations are consistent with the hypothesis that the rate limiting steps for both lag and growth phases are apparent first order with respect to collagen concentration.
|Original language||English (US)|
|Number of pages||13|
|Journal||Topics in Catalysis|
|State||Published - 1983|
All Science Journal Classification (ASJC) codes
- N-tris-hydroxymethyl methyl2-aminoethane sulfonic acid.
- collagen fibrillogenesis
- kinetic analysis
- light scattering
- sodium dodecyl sulfate
- turbidity-time curve