TY - JOUR
T1 - L1 mono- And polyclonal antibodies modify cell migration in early postnatal mouse cerebellum
AU - Lindner, J.
AU - Rathjen, F. G.
AU - Schachner, M.
PY - 1983
Y1 - 1983
N2 - A major event of nervous system development1 is the migration of granule cell neurones, during the early postnatal development of the cerebellar cortex, from their germinating zone in the external granular layer to then final location in the internal granular layer. During migration, many granule cells are seen in direct cell-surface contact with processes of Bergmann glia, a subclass of astrocytes2. In the neurological mutant mouse weaver, however, migration of granule cells is impaired, probably due to a deficit in cell-cell interactions3-5. To gain insight into the cellular and molecular mechanisms involved in granule cell migration, we have used a modification of an in vitro assay system, previously described by Moonen et al.6, which displays migratory behaviour in small tissue explants during several days of suspension culture. The aim of this study was to investigate the process of granule cell migration by using antibodies directed against cell-surface components of developing neural cells. We report here that migration of 3H-thymidine-labelled granule cell neurones can be modified by Fab fragments of both mono- and polyclonal L1 antibodies, but not by Fab fragments of polyclonal antibodies prepared against mouse liver membranes, which also react with cerebellar cell surfaces.
AB - A major event of nervous system development1 is the migration of granule cell neurones, during the early postnatal development of the cerebellar cortex, from their germinating zone in the external granular layer to then final location in the internal granular layer. During migration, many granule cells are seen in direct cell-surface contact with processes of Bergmann glia, a subclass of astrocytes2. In the neurological mutant mouse weaver, however, migration of granule cells is impaired, probably due to a deficit in cell-cell interactions3-5. To gain insight into the cellular and molecular mechanisms involved in granule cell migration, we have used a modification of an in vitro assay system, previously described by Moonen et al.6, which displays migratory behaviour in small tissue explants during several days of suspension culture. The aim of this study was to investigate the process of granule cell migration by using antibodies directed against cell-surface components of developing neural cells. We report here that migration of 3H-thymidine-labelled granule cell neurones can be modified by Fab fragments of both mono- and polyclonal L1 antibodies, but not by Fab fragments of polyclonal antibodies prepared against mouse liver membranes, which also react with cerebellar cell surfaces.
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U2 - 10.1038/305427a0
DO - 10.1038/305427a0
M3 - Article
C2 - 6621692
AN - SCOPUS:0021046979
SN - 0028-0836
VL - 305
SP - 427
EP - 430
JO - Nature
JF - Nature
IS - 5933
ER -