Abstract
Thin ex vivo biological specimens, such as single cells and tissue slices, are widely used in biology and medicine. Optical microscopic imaging and spectroscopic techniques are ideal tools for probing the composition of these specimens nondestructively and with minimal sample preparation and handling. This chapter focuses on elastic scattering as one of these optically based analytical techniques. Unlike most turbid tissues analyzed in vivo, light scattering in these relatively thin ex vivo specimens is dominated by single scattering. Here, we describe the methods currently used to collect and interpret elastic scattering data from thin biological specimens, and biomedical applications in this field.
| Original language | English (US) |
|---|---|
| Title of host publication | Biomedical Photonics |
| Subtitle of host publication | Handbook |
| Publisher | CRC Press |
| Pages | 16-1-16-23 |
| ISBN (Electronic) | 9780203008997 |
| ISBN (Print) | 0849311160, 9780849311161 |
| State | Published - Jan 1 2003 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- General Medicine
- General Biochemistry, Genetics and Molecular Biology
- General Physics and Astronomy