Limited ERG11 mutations identified in isolates of candida auris directly contribute to reduced azole susceptibility

Kelley R. Healey; Milena Kordalewska; Cristina Jiménez Ortigosa; Ashutosh Singh; Indira Berrío; Anuradha Chowdhary; David S. Perlin

doi:10.1128/AAC.01427-18

Limited ERG11 mutations identified in isolates of candida auris directly contribute to reduced azole susceptibility

Kelley R. Healey, Milena Kordalewska, Cristina Jiménez Ortigosa, Ashutosh Singh, Indira Berrío, Anuradha Chowdhary, David S. Perlin

New Jersey Medical School (NJMS), Public Health Research Institute Center

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. auris ERG11 alleles that encoded for Y132F or K143R substitutions; however, expression of alleles encoding I466M, Y501H, or other clade-defined amino acid differences yielded susceptible MICs. Similar to other Candida species, specific C. auris ERG11 mutations resulted directly in reduced azole susceptibility.

Original language	English (US)
Article number	e01427
Journal	Antimicrobial agents and chemotherapy
Volume	62
Issue number	10
DOIs	https://doi.org/10.1128/AAC.01427-18
State	Published - Oct 2018

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)
Infectious Diseases

Keywords

Antifungal resistance
Azoles
Candida auris
ERG11
Reduced azole susceptibility

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10.1128/AAC.01427-18

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abstract = "Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. auris ERG11 alleles that encoded for Y132F or K143R substitutions; however, expression of alleles encoding I466M, Y501H, or other clade-defined amino acid differences yielded susceptible MICs. Similar to other Candida species, specific C. auris ERG11 mutations resulted directly in reduced azole susceptibility.",

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T1 - Limited ERG11 mutations identified in isolates of candida auris directly contribute to reduced azole susceptibility

AU - Healey, Kelley R.

AU - Kordalewska, Milena

AU - Ortigosa, Cristina Jiménez

AU - Singh, Ashutosh

AU - Berrío, Indira

AU - Chowdhary, Anuradha

AU - Perlin, David S.

PY - 2018/10

Y1 - 2018/10

N2 - Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. auris ERG11 alleles that encoded for Y132F or K143R substitutions; however, expression of alleles encoding I466M, Y501H, or other clade-defined amino acid differences yielded susceptible MICs. Similar to other Candida species, specific C. auris ERG11 mutations resulted directly in reduced azole susceptibility.

AB - Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. auris ERG11 alleles that encoded for Y132F or K143R substitutions; however, expression of alleles encoding I466M, Y501H, or other clade-defined amino acid differences yielded susceptible MICs. Similar to other Candida species, specific C. auris ERG11 mutations resulted directly in reduced azole susceptibility.

KW - Antifungal resistance

KW - Azoles

KW - Candida auris

KW - ERG11

KW - Reduced azole susceptibility

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Limited ERG11 mutations identified in isolates of candida auris directly contribute to reduced azole susceptibility

Abstract

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