Linking specific heterotrophic bacterial populations to bioreduction of uranium and nitrate in contaminated subsurface sediments by using stable isotope probing

Denise M. Akob; Lee Kerkhof; Kirsten Küsel; David B. Watson; Anthony V. Palumbo; Joel E. Kostka

doi:10.1128/AEM.05247-11

Linking specific heterotrophic bacterial populations to bioreduction of uranium and nitrate in contaminated subsurface sediments by using stable isotope probing

Denise M. Akob, Lee Kerkhof, Kirsten Küsel, David B. Watson, Anthony V. Palumbo, Joel E. Kostka

School of Environmental and Biological Sciences, Marine & Coastal Sciences

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [ ¹³C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.

Original language	English (US)
Pages (from-to)	8197-8200
Number of pages	4
Journal	Applied and environmental microbiology
Volume	77
Issue number	22
DOIs	https://doi.org/10.1128/AEM.05247-11
State	Published - Nov 2011

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [ 13C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.",

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AU - Akob, Denise M.

AU - Kerkhof, Lee

AU - Küsel, Kirsten

AU - Watson, David B.

AU - Palumbo, Anthony V.

AU - Kostka, Joel E.

PY - 2011/11

Y1 - 2011/11

N2 - Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [ 13C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.

AB - Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [ 13C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.

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ER -

Linking specific heterotrophic bacterial populations to bioreduction of uranium and nitrate in contaminated subsurface sediments by using stable isotope probing

Abstract

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