In this study, we identified and quantitated the lipid components in the secretions of human bronchial explants cultured in a serum-free medium over a period of 50 days. Total lipids represented 6% of the dry material. This amount is considerably lower than that reported for 'normal' human sputum and suggests that the latter is contaminated by serum transudates, alveolar secretions, and microorganisms. Such serum-free culture systems are highly suitable to study cell physiology as its relates to human disease.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- 4-(2-hydroxyethyl)-1-piperzineethane-sulfonic acid
- Lipid composition
- Lung secretion