Localisation of cardiac G(Sα) in transgenic mice overexpressing G(Sα)

Kathryn H. Muntz, Yoshihiro Ishikawa, Thomas Wagner, Charles J. Homcy, Stephen F. Vatner, Dorothy E. Vatner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The biochemical and physiological effects of G(Sα) activation are well known; however, little is known about the anatomical localisation of G(Sα) in the myocardium. Knowledge of the localisation might yield insights into G protein function in heart. The utility of immunocytochemistry using immunofluorescent methods is limited in normal hearts because of the low expression of G(Sα). In order to magnify the G(Sα) signal, we studied transgenic mice overexpressing myocardial G(Sα). Immunofluorescent techniques with confocal imaging using rabbit antiserum specific for G(Sα) were studied in frozen sections of mouse left ventricle G(Sα) labeling appeared to be localised to the T-tubules and intercalated disks in the G(Sα) overexpressing mouse hearts, whereas the control mice showed background fluorescence with diffuse faint labeling. The localisation of G(Sα) to structures involved in calcium handling and membrane conductance places G(Sα) at a focal point in the regulation of these key functions.

Original languageEnglish (US)
Pages (from-to)1649-1653
Number of pages5
JournalJournal of Molecular and Cellular Cardiology
Volume29
Issue number6
DOIs
StatePublished - Jun 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Keywords

  • Confocal imaging
  • Immunofluorescence
  • Intercalated disk
  • T-tubule

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