Localization and requirement for myosin II at the dorsal-ventral compartment boundary of the Drosophila wing

Robert J. Major, Kenneth D. Irvine

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

As organisms develop, their tissues can become separated into distinct cell populations through the establishment of compartment boundaries. Compartment boundaries have been discovered, in a wide variety of tissues, but in many cases the molecular mechanisms that separate cells remain poorly understood. In the Drosophila wing, a stripe of Notch activation maintains the dorsal-ventral compartment boundary, through a process that depends on the actin cytoskeleton. Here, we show that the dorsal-ventral boundary exhibits a distinct accumulation of Myosin II, and that this accumulation is regulated downstream of Notch signaling. Conversely, the dorsal-ventral boundary is depleted for the Par-3 homologue Bazooka. We further show that mutations in the Myosin heavy chain subunit encoded by zipper can impair dorsal-ventral compartmentalization without affecting anterior-posterior compartmentalization. These observations identify a distinct accumulation and requirement for Myosin activity in dorsal-ventral compartmentalization, and suggest a novel mechanism in which contractile tension along an F-actin cable at the compartment boundary contributes to compartmentalization.

Original languageEnglish (US)
Pages (from-to)3051-3058
Number of pages8
JournalDevelopmental Dynamics
Volume235
Issue number11
DOIs
StatePublished - Nov 2006

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Keywords

  • Actin
  • Compartment
  • Drosophila
  • Myosin
  • Notch
  • Wing

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