Localization of a putative inositol 1,4,5-triphosphate receptor in the Limulus granulocyte

Eric Solon, Ayodhya P. Gupta, Randy Gaugler

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The horseshoe crab (Limulus polyphemus) granulocyte (GR) degranulates upon contact with bacteria and release factors that mediate an immune response. Stimulated cells produce IP3, which binds to receptors (IP3R, M.W. 240-300 kD) that function to release stored Ca2+ into the cytoplasm that mediates degranulation. This mechanism is believed to mediate exocytosis in the Limulus GR but IP3R in the GR has not been shown. The present study utilized monoclonal antibody 4C11 and a commercially available antiIP3R antibody, both of which label amino acids of the N-terminal of all known isoforms. Electron microscopy, immunohistochemistry, SDS-PAGE, and Western blot analysis, which employed the use of the two antibodies, demonstrates that a putative IP3R exists in the: plasma membrane, smooth surfaced vesicles, nucleus and nuclear membrane. We hypothesize that this putative IP3R is involved in mediating the immune response of the Limulus GR.

Original languageEnglish (US)
Pages (from-to)277-285
Number of pages9
JournalDevelopmental and Comparative Immunology
Issue number3
StatePublished - May 1997

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology


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