Location and magnification of 5 S RNA genes in Saccharomyces cerevisiae

David Kaback, Harlyn O. Halvorson, Gerald M. Rubin

Research output: Contribution to journalLetter

3 Citations (Scopus)

Abstract

We have compared the amount of DNA complementary to the 5 S, 5·8 S and 4 S RNAs in a strain of Saccharomyces cerevisiae monosomic for chromosome I and a related diploid. The monosomic strain contains approximately 25% less DNA complementary to both 5 S and 5·8 S RNA than the diploid. The amount of DNA complementary to 4 S RNA is the same in both strains. These results indicate that the same relative proportions of the 5 S RNA genes, and the 5·8 S genes as well as the 18 S and 26 S RNA genes are present on chromosome I. Moreover, we have observed that the monosomic strain reverted to normal levels of DNA complementary to 5 S, 5·8 S, 18 S and 26 S RNA when continuously cultured for several months even though the strain remained monosomic for chromosome I by genetic criteria. This co-ordinate recovery in gene number is consistent with the close association of the 5 S, 5·8 S, 18 S and 26 S ribosomal RNA genes in yeast.

Original languageEnglish (US)
Pages (from-to)385-390
Number of pages6
JournalJournal of molecular biology
Volume107
Issue number3
DOIs
StatePublished - Nov 5 1976
Externally publishedYes

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Saccharomyces cerevisiae
RNA
Complementary DNA
Genes
Chromosomes
Diploidy
rRNA Genes
Yeasts

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Kaback, David ; Halvorson, Harlyn O. ; Rubin, Gerald M. / Location and magnification of 5 S RNA genes in Saccharomyces cerevisiae. In: Journal of molecular biology. 1976 ; Vol. 107, No. 3. pp. 385-390.
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abstract = "We have compared the amount of DNA complementary to the 5 S, 5·8 S and 4 S RNAs in a strain of Saccharomyces cerevisiae monosomic for chromosome I and a related diploid. The monosomic strain contains approximately 25{\%} less DNA complementary to both 5 S and 5·8 S RNA than the diploid. The amount of DNA complementary to 4 S RNA is the same in both strains. These results indicate that the same relative proportions of the 5 S RNA genes, and the 5·8 S genes as well as the 18 S and 26 S RNA genes are present on chromosome I. Moreover, we have observed that the monosomic strain reverted to normal levels of DNA complementary to 5 S, 5·8 S, 18 S and 26 S RNA when continuously cultured for several months even though the strain remained monosomic for chromosome I by genetic criteria. This co-ordinate recovery in gene number is consistent with the close association of the 5 S, 5·8 S, 18 S and 26 S ribosomal RNA genes in yeast.",
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Location and magnification of 5 S RNA genes in Saccharomyces cerevisiae. / Kaback, David; Halvorson, Harlyn O.; Rubin, Gerald M.

In: Journal of molecular biology, Vol. 107, No. 3, 05.11.1976, p. 385-390.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Location and magnification of 5 S RNA genes in Saccharomyces cerevisiae

AU - Kaback, David

AU - Halvorson, Harlyn O.

AU - Rubin, Gerald M.

PY - 1976/11/5

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N2 - We have compared the amount of DNA complementary to the 5 S, 5·8 S and 4 S RNAs in a strain of Saccharomyces cerevisiae monosomic for chromosome I and a related diploid. The monosomic strain contains approximately 25% less DNA complementary to both 5 S and 5·8 S RNA than the diploid. The amount of DNA complementary to 4 S RNA is the same in both strains. These results indicate that the same relative proportions of the 5 S RNA genes, and the 5·8 S genes as well as the 18 S and 26 S RNA genes are present on chromosome I. Moreover, we have observed that the monosomic strain reverted to normal levels of DNA complementary to 5 S, 5·8 S, 18 S and 26 S RNA when continuously cultured for several months even though the strain remained monosomic for chromosome I by genetic criteria. This co-ordinate recovery in gene number is consistent with the close association of the 5 S, 5·8 S, 18 S and 26 S ribosomal RNA genes in yeast.

AB - We have compared the amount of DNA complementary to the 5 S, 5·8 S and 4 S RNAs in a strain of Saccharomyces cerevisiae monosomic for chromosome I and a related diploid. The monosomic strain contains approximately 25% less DNA complementary to both 5 S and 5·8 S RNA than the diploid. The amount of DNA complementary to 4 S RNA is the same in both strains. These results indicate that the same relative proportions of the 5 S RNA genes, and the 5·8 S genes as well as the 18 S and 26 S RNA genes are present on chromosome I. Moreover, we have observed that the monosomic strain reverted to normal levels of DNA complementary to 5 S, 5·8 S, 18 S and 26 S RNA when continuously cultured for several months even though the strain remained monosomic for chromosome I by genetic criteria. This co-ordinate recovery in gene number is consistent with the close association of the 5 S, 5·8 S, 18 S and 26 S ribosomal RNA genes in yeast.

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U2 - 10.1016/S0022-2836(76)80012-5

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JF - Journal of Molecular Biology

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