Location and magnification of 5 S RNA genes in Saccharomyces cerevisiae

David B. Kaback, Harlyn O. Halvorson, Gerald M. Rubin

Research output: Contribution to journalLetter

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Abstract

We have compared the amount of DNA complementary to the 5 S, 5·8 S and 4 S RNAs in a strain of Saccharomyces cerevisiae monosomic for chromosome I and a related diploid. The monosomic strain contains approximately 25% less DNA complementary to both 5 S and 5·8 S RNA than the diploid. The amount of DNA complementary to 4 S RNA is the same in both strains. These results indicate that the same relative proportions of the 5 S RNA genes, and the 5·8 S genes as well as the 18 S and 26 S RNA genes are present on chromosome I. Moreover, we have observed that the monosomic strain reverted to normal levels of DNA complementary to 5 S, 5·8 S, 18 S and 26 S RNA when continuously cultured for several months even though the strain remained monosomic for chromosome I by genetic criteria. This co-ordinate recovery in gene number is consistent with the close association of the 5 S, 5·8 S, 18 S and 26 S ribosomal RNA genes in yeast.

Original languageEnglish (US)
Pages (from-to)385-390
Number of pages6
JournalJournal of molecular biology
Volume107
Issue number3
DOIs
StatePublished - Nov 5 1976
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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