Location of structural transitions in an isotopically labeled lung surfactant SP-B peptide by IRRAS

Carol R. Flach, Peng Cai, Darline Dieudonné, Joseph W. Brauner, Kevin M.W. Keough, June Stewart, Richard Mendelsohn

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Pulmonary surfactant, a lipid/protein complex that lines the air/water interface in the mammalian lung, functions to reduce the work of breathing. Surfactant protein B (SP-B) is a small, hydrophobic protein that is an essential component of this mixture. Structure-function relationships of SP-B are currently under investigation as the protein and its peptide analogs are being incorporated into surfactant replacement therapies. Knowledge of the structure of SP-B and its related peptides in bulk and monolayer phases will facilitate the design of later generation therapeutic agents. Prior infrared reflection-absorption spectroscopic studies reported notable, reversible surface pressure-induced antiparallel β-sheet formation in a synthetic peptide derived from human SP-B, residues 9-36 (SP-B9-36). In the current work, infrared reflection-absorption spectroscopy is applied in conjunction with isotopic labeling to detect the site and pressure dependence of the conformational change. SP-B9-36, synthesized with 13C=O-labeled Ala residues in positions 26, 28, 30, and 32, shifted the β-sheet marker band to ∼1600 cm-1 and thus immediately identified this structural element within the labeled region. Surface pressure-induced alterations in the relative intensities of Amide I band constituents are interpreted using a semiempirical transition dipole coupling model. In addition, electron micrographs reveal the formation of tubular myelin structures from in vitro preparations using SP-B9-36 in place of porcine SP-B indicating that the peptide has the potential to mimic this property of the native protein.

Original languageEnglish (US)
Pages (from-to)340-349
Number of pages10
JournalBiophysical Journal
Volume85
Issue number1
DOIs
StatePublished - Jul 1 2003

All Science Journal Classification (ASJC) codes

  • Biophysics

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