Abstract
The goal of this study is to investigate the effect of chronic exposure to inflammatory mediators on cultured hepatocytes as a model of inflammation-induced hepatic dysfunction. A collagen sandwich culture model capable of preserving liverspecific functions of adult rat hepatocytes for 6-8 weeks is used. Briefly, exposure to IL-1beta suppressed hepatocytes protein synthesis as measured by albumin and fibrinogen secretion. IL-6 initially suppressed albumin synthesis, but the effect gradually wore off after 2 weeks despite the continuous exposure. Combination of IL-1beta and IL-6 showed same result as IL-1beta alone suggesting that the inhibitory effect of IL-1beta dominated over any effects of IL-6. IL-1beta and IL-6 caused suppression of urea production.
| Original language | English (US) |
|---|---|
| Pages (from-to) | S-82 |
| Journal | Annals of Biomedical Engineering |
| Volume | 28 |
| Issue number | SUPPL. 1 |
| State | Published - 2000 |
| Externally published | Yes |
| Event | 2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA Duration: Oct 12 2000 → Oct 14 2000 |
All Science Journal Classification (ASJC) codes
- Biomedical Engineering