Long-term potentiation in vivo increases rat hippocampal tenascin-C expression

Marina Nakic, Denise Manahan-Vaughan, Klaus G. Reymann, Melitta Schachner

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We investigated the expression of the extracellular matrix glycoprotein tenascin-C after induction of long-term potentiation (LTP) by high-frequency tetanization (HFT) in the rat dentate gyrus in vivo. Expression of tenascin- C was evaluated at the mRNA and protein levels by in situ hybridization and immunocytochemistry, respectively. Whereas no tenascin-C mRNA was detectable in control animals, an increase in tenascin-C mRNA levels was observed in the granule cell layer of the dentate gyrus 4 h after HFT. At 24 h after HFT, tenascin-C mRNA had returned to control levels. Expression of tenascin-C protein 4 h after HFT followed that of controls in that tenascin was detectable in the strata oriens and radiatum of CA1, in the molecular layer, and within a narrow area at the inner surface of the granule cell layer in the dentate gyrus. However, 24 h after HFT, additional patches of tenascin-C immunoreactivity were observed in the molecular layer of the dentate gyrus. No increase in tenascin mRNA or protein levels was detected in control animals that received no stimulation, low-frequency stimulation, or HFT in the presence of the N-methyl-n-aspartate receptor antagonist D(-)-2-amino-5- phosphonopentanoic acid or the metabotropic glutamate receptor antagonist (R,S)-α-methyl-4-carboxyphenylglycine. These observations implicate a role for tenascin-C in N-methyl-D-aspartate and metabotropic glutamate receptor- dependent changes accompanying induction and/or maintenance of LTP.

Original languageEnglish (US)
Pages (from-to)393-404
Number of pages12
JournalJournal of Neurobiology
Volume37
Issue number3
DOIs
StatePublished - Nov 15 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Keywords

  • Extracellular matrix
  • MGluR
  • NMDA
  • Recognition molecule
  • Synaptic plasticity

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