Long time-lapse imaging reveals unique features of PARK2/Parkin-mediated mitophagy in mature cortical neurons

Qian Cai, Hesham Mostafa Zakaria, Zu Hang Sheng

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Proper degradation of aged and damaged mitochondria through mitophagy is essential to ensure mitochondrial integrity and function. Translocation of PARK2/Parkin onto damaged mitochondria induces mitophagy in many non-neuronal cell types. However, direct evidence showing PARK2-mediated mitophagy in mature neurons is controversial, leaving unanswered questions as to how, where, and by what time course PARK2-mediated mitophagy occurs in neurons following mitochondrial depolarization. We applied long time-lapse imaging in live mature cortical neurons to monitor the slow but dynamic and spatial PARK2 translocation onto damaged mitochondria and subsequent degradation through the autophagy-lysosomal pathway. In comparison with non-neuronal cells, our study reveals unique features of PARK2-mediated mitophagy in mature neurons, which will advance our understanding of pathogenesis of several major neurodegenerative diseases characterized by damaged mitochondria or a dysfunctional autophagy-lysosomal system.

Original languageEnglish (US)
Pages (from-to)976-978
Number of pages3
JournalAutophagy
Volume8
Issue number6
DOIs
StatePublished - Jun 2012

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Keywords

  • Autophagosome
  • Autophagy
  • Depolarization
  • Lysosome
  • Mitochondria
  • Mitochondrial membrane potential
  • Mitochondrial mobility
  • Mitochondrial quality control
  • Neuronal mitophagy
  • PARK2
  • Parkin

Fingerprint Dive into the research topics of 'Long time-lapse imaging reveals unique features of PARK2/Parkin-mediated mitophagy in mature cortical neurons'. Together they form a unique fingerprint.

  • Cite this