TY - JOUR
T1 - Lsd1 and Lsd2 Control Programmed Replication Fork Pauses and Imprinting in Fission Yeast
AU - Holmes, Allyson
AU - Roseaulin, Laura
AU - Schurra, Catherine
AU - Waxin, Herve
AU - Lambert, Sarah
AU - Zaratiegui, Mikel
AU - Martienssen, Robert A.
AU - Arcangioli, Benoit
N1 - Funding Information:
We thank the scientific community for yeast strains: in particular, J.Z. Dalgaard for SS2; F. Lan and Y. Shi for lsd1-ao and lsd2-ao ; K. Ekwall, X. Sun, and J. Pelloux for their help; and J. Cooper for critical reading of the manuscript. This work was supported by grants from the ARC and conseil regional de Martinique to L.R. and ANR-06-BLAN-0271 to S.L. and B.A.
PY - 2012/12/27
Y1 - 2012/12/27
N2 - In the fission yeast Schizosaccharomyces pombe, a chromosomal imprinting event controls the asymmetric pattern of mating-type switching. The orientation of DNA replication at the mating-type locus is instrumental in this process. However, the factors leading to imprinting are not fully identified and the mechanism is poorly understood. Here, we show that the replication fork pause at the mat1 locus (MPS1), essential for imprint formation, depends on the lysine-specific demethylase Lsd1. We demonstrate that either Lsd1 or Lsd2 amine oxidase activity is required for these processes, working upstream of the imprinting factors Swi1 and Swi3 (homologs of mammalian Timeless and Tipin, respectively). We also show that the Lsd1/2 complex controls the replication fork terminators, within the rDNA repeats. These findings reveal a role for the Lsd1/2 demethylases in controlling polar replication fork progression, imprint formation, and subsequent asymmetric cell divisions.
AB - In the fission yeast Schizosaccharomyces pombe, a chromosomal imprinting event controls the asymmetric pattern of mating-type switching. The orientation of DNA replication at the mating-type locus is instrumental in this process. However, the factors leading to imprinting are not fully identified and the mechanism is poorly understood. Here, we show that the replication fork pause at the mat1 locus (MPS1), essential for imprint formation, depends on the lysine-specific demethylase Lsd1. We demonstrate that either Lsd1 or Lsd2 amine oxidase activity is required for these processes, working upstream of the imprinting factors Swi1 and Swi3 (homologs of mammalian Timeless and Tipin, respectively). We also show that the Lsd1/2 complex controls the replication fork terminators, within the rDNA repeats. These findings reveal a role for the Lsd1/2 demethylases in controlling polar replication fork progression, imprint formation, and subsequent asymmetric cell divisions.
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U2 - 10.1016/j.celrep.2012.10.011
DO - 10.1016/j.celrep.2012.10.011
M3 - Article
C2 - 23260662
AN - SCOPUS:84871707126
SN - 2211-1247
VL - 2
SP - 1513
EP - 1520
JO - Cell Reports
JF - Cell Reports
IS - 6
ER -