Maf family transcription factors are required for nutrient uptake in the mouse neonatal gut

Anne M. Bara, Lei Chen, Celina Ma, Julie Underwood, Rebecca S. Moreci, Kaelyn Sumigray, Tongyu Sun, Yarui Diao, Michael Verzi, Terry Lechler

Research output: Contribution to journalArticlepeer-review


There are fundamental differences in howneonatal and adult intestines absorb nutrients. In adults, macromolecules are broken down into simplermolecular components in the lumen of the small intestine, then absorbed. In contrast, neonates are thought to rely on internalization of whole macromolecules and subsequent degradation in the lysosome. Here, we identify the Maf family transcription factors MAFB and c-MAF asmarkers of terminally differentiated intestinal enterocytes throughout life. The expression of these factors is regulated byHNF4α andHNF4γ, master regulators of enterocyte cell fate. Loss of Maf factors results in a neonatal-specific failure to thrive and loss of macromolecular nutrient uptake. RNA-Seq and CUT&RUN analyses defined an endolysosomal program as being downstreamof these transcription factors. We demonstrate major transcriptional changes in metabolic pathways, including fatty acid oxidation and increases in peroxisome number, in response to loss of Maf proteins. Finally, we show that loss of BLIMP1, a repressor of adult enterocyte genes, shows highly overlapping changes in gene expression and similar defects in macromolecular uptake. This work defines transcriptional regulators that are necessary for nutrient uptake in neonatal enterocytes.

Original languageEnglish (US)
Article numberdev201251
JournalDevelopment (Cambridge)
Issue number23
StatePublished - Dec 2022

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology


  • Enterocyte
  • Intestine
  • Lysosomes
  • Mouse
  • Neonatal
  • Uptake


Dive into the research topics of 'Maf family transcription factors are required for nutrient uptake in the mouse neonatal gut'. Together they form a unique fingerprint.

Cite this