Magnitudes and Orientations of the 15N Chemical Shift Tensor of [1-15N]-2′-Deoxyguanosine Determined on a Polycrystalline Sample by Two-Dimensional Solid-State NMR Spectroscopy

Gary A. Lorigan, Ronald McNamara, Roger A. Jones, Stanley J. Opella

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The magnitudes and orientations of the 15N chemical shift tensor of [1-15N]-2′-deoxyguanosine were determined from a polycrystalline sample using the two-dimensional PISEMA experiment. The magnitudes of the principal values of the 15N chemical shift tensor of the N1 nitrogen of [1-15N]-2′-deoxyguanosine were found to be σ11 = 54 ppm, σ22 = 148 ppm, and σ33 = 201 ppm with respect to (15NH4)2SO4 in aqueous solution. Comparisons of experimental and simulated two-dimensional powder pattern spectra show that σ33N is approximately collinear with the N-H bond. The tensor orientation of σ33N for N1 of [1-15N]-2′-deoxyguanosine is similar to the values obtained for the side chain residues of 15N∈1-tryptophan and 15Nπ-histidine even though the magnitudes differ significantly.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalJournal of Magnetic Resonance
Volume140
Issue number2
DOIs
StatePublished - Oct 1999

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Keywords

  • DNA
  • Deoxyguanosine
  • N chemical shift tensor
  • Solid-state NMR

Fingerprint Dive into the research topics of 'Magnitudes and Orientations of the <sup>15</sup>N Chemical Shift Tensor of [1-<sup>15</sup>N]-2′-Deoxyguanosine Determined on a Polycrystalline Sample by Two-Dimensional Solid-State NMR Spectroscopy'. Together they form a unique fingerprint.

Cite this