Major trauma, enhances store-operated calcium influx in human neutrophils

Carl J. Hauser, Zoltan Fekete, David H. Livingston, John Adams, Matthew Garced, Edwin A. Deitch

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Purpose: Chemotaxins from inflammatory sites prime or activate neutrophils (PMN) by using cytosolic calcium ([Ca2+](i)) fluxes as second messengers. [Ca2+](i) can be mobilized rapidly by receptor-mediated entry or store-release, or more slowly by store-operated calcium influx (SOCI). We studied [Ca2+](i) mobilization by chemotaxins and how trauma impacts the calcium entry mechanisms used by chemotaxins. Methods: [Ca2+](i) flux was studied by spectrofluorometry. The contributions of early and late [Ca2+](i) currents to net calcium flux were compared after stimulation by more potent (fMLP, C5a, PAF) or less potent (IL-8, GRO-α, and LTB4) agonists. Store operated [Ca2+](i) mobilization was reflected by the ratio of area under the [Ca2+](i) efflux curve to peak [Ca2+](i) (efflux curve). PMN from trauma patients (ISS > 25) and pair-matched volunteer (n = 7 pairs) were then primed and stimulated with thapsigargin to compare cell calcium stores and SOCI. Results: Late [Ca2+](i) mobilization made more important contributions to fMLP, PAF, and C5a signals than to IL-8, GRO-α, or LTB4 (p < 0.01 all comparisons). Calcium stores and store release were only marginally lower after injury (p = not significant), but trauma PMN showed far higher [Ca2+](i) influx after thapsigargin (p = 0.007), and greater net SOCI (p = 0.034). Conclusions: SOCI may play an important role in PMN activation, and trauma increases PMN SOCI. Prolonged elevations of [Ca2+](i) due to enhanced SOCI may alter stimulus-response coupling to chemotaxins and contribute to PMN disfunction after injury.

Original languageEnglish (US)
Pages (from-to)592-598
Number of pages7
JournalJournal of Trauma - Injury, Infection and Critical Care
Issue number4
StatePublished - Apr 2000

All Science Journal Classification (ASJC) codes

  • Surgery
  • Critical Care and Intensive Care Medicine


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