TY - JOUR
T1 - Mapping of RNA Polymerase Residues that Interact with Bacteriophage Xp10 Transcription Antitermination Factor p7
AU - Yuzenkova, Yulia
AU - Zenkin, Nikolay
AU - Severinov, Konstantin
N1 - Funding Information:
This work was supported by NIH GM grant R01 59295, Russian Academy of Sciences Presidium program in Molecular and Cellular Biology new groups grant, and Burroughs Wellcome Career Award in Biomedical Sciences to (K.S.).
PY - 2008/1/4
Y1 - 2008/1/4
N2 - Bacteriophage Xp10-encoded transcription factor p7 interacts with host Xanthomonas oryzae RNA polymerase β′ subunit and prevents both promoter recognition by the RNA polymerase holoenzyme and transcription termination by the RNA polymerase core. P7 does not bind to and has no effect on RNA polymerase from Escherichia coli. Here, we use a combination of biochemical and genetic methods to map the p7 interaction site to within four β′ amino acid residues at the N terminus of X. oryzae RNAP β′. The interaction site is located in an area that is close to the promoter spacer in the open complex and to the upstream boundary of the transcription bubble in the elongation complex, providing a possible explanation of how a small protein can affect both transcription initiation and termination by binding to the same RNA polymerase site.
AB - Bacteriophage Xp10-encoded transcription factor p7 interacts with host Xanthomonas oryzae RNA polymerase β′ subunit and prevents both promoter recognition by the RNA polymerase holoenzyme and transcription termination by the RNA polymerase core. P7 does not bind to and has no effect on RNA polymerase from Escherichia coli. Here, we use a combination of biochemical and genetic methods to map the p7 interaction site to within four β′ amino acid residues at the N terminus of X. oryzae RNAP β′. The interaction site is located in an area that is close to the promoter spacer in the open complex and to the upstream boundary of the transcription bubble in the elongation complex, providing a possible explanation of how a small protein can affect both transcription initiation and termination by binding to the same RNA polymerase site.
KW - RNA polymerase
KW - bacteriophage
KW - promoter recognition
KW - transcription antitermination
KW - transcription factor
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U2 - 10.1016/j.jmb.2007.10.054
DO - 10.1016/j.jmb.2007.10.054
M3 - Article
C2 - 18021805
AN - SCOPUS:36348959131
VL - 375
SP - 29
EP - 35
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -