Mapping of the region of the tick-borne encephalitis virus replicase adjacent to initiating substrate binding center

O. V. Morozova; A. A. Mustaev; N. A. Belyavskaya; E. F. Zaychikov; E. A. Kvetkova; Yu I. Wolf; A. G. Pletnev

doi:10.1016/0014-5793(90)80812-W

Mapping of the region of the tick-borne encephalitis virus replicase adjacent to initiating substrate binding center

O. V. Morozova, A. A. Mustaev, N. A. Belyavskaya, E. F. Zaychikov, E. A. Kvetkova, Yu I. Wolf, A. G. Pletnev

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Affinity labelling with aldehyde-containing analogs of initiation substrates of nuclear fraction of tick-borne encephalitis virus (TBEV) infected cells results in a labelling of a single polypeptide with a molecular mass of 68 kDa which was immunologically identified as TBEV NS3 protein. A single-hit hydroxylamine hydrolysis, using limited and long-term CNBr cleavages allowed one to identify Lys¹⁸⁰⁰ and/or Lys¹⁸⁰³ as the label attachment sites. These amino acid residues are situated in the proximity of the 'B'-site of NTP-binding motif of viral RNA replicase.

Original language	English (US)
Pages (from-to)	75-77
Number of pages	3
Journal	FEBS Letters
Volume	277
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)80812-W
State	Published - Dec 17 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Keywords

Active center
Affinity labelling
Flavivirus
Replicase

Access to Document

10.1016/0014-5793(90)80812-W

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Cite this

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title = "Mapping of the region of the tick-borne encephalitis virus replicase adjacent to initiating substrate binding center",

abstract = "Affinity labelling with aldehyde-containing analogs of initiation substrates of nuclear fraction of tick-borne encephalitis virus (TBEV) infected cells results in a labelling of a single polypeptide with a molecular mass of 68 kDa which was immunologically identified as TBEV NS3 protein. A single-hit hydroxylamine hydrolysis, using limited and long-term CNBr cleavages allowed one to identify Lys1800 and/or Lys1803 as the label attachment sites. These amino acid residues are situated in the proximity of the 'B'-site of NTP-binding motif of viral RNA replicase.",

keywords = "Active center, Affinity labelling, Flavivirus, Replicase",

author = "Morozova, {O. V.} and Mustaev, {A. A.} and Belyavskaya, {N. A.} and Zaychikov, {E. F.} and Kvetkova, {E. A.} and Wolf, {Yu I.} and Pletnev, {A. G.}",

year = "1990",

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doi = "10.1016/0014-5793(90)80812-W",

language = "English (US)",

volume = "277",

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T1 - Mapping of the region of the tick-borne encephalitis virus replicase adjacent to initiating substrate binding center

AU - Morozova, O. V.

AU - Mustaev, A. A.

AU - Belyavskaya, N. A.

AU - Zaychikov, E. F.

AU - Kvetkova, E. A.

AU - Wolf, Yu I.

AU - Pletnev, A. G.

PY - 1990/12/17

Y1 - 1990/12/17

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AB - Affinity labelling with aldehyde-containing analogs of initiation substrates of nuclear fraction of tick-borne encephalitis virus (TBEV) infected cells results in a labelling of a single polypeptide with a molecular mass of 68 kDa which was immunologically identified as TBEV NS3 protein. A single-hit hydroxylamine hydrolysis, using limited and long-term CNBr cleavages allowed one to identify Lys1800 and/or Lys1803 as the label attachment sites. These amino acid residues are situated in the proximity of the 'B'-site of NTP-binding motif of viral RNA replicase.

KW - Active center

KW - Affinity labelling

KW - Flavivirus

KW - Replicase

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