Abstract
Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily observed. Although several biochemical and morphological parameters have been described for various types of cell death in plants, the relationships existing between those different types of PCD events remain unclear. In this work, we set out to examine if two early molecular markers of HR cell death (HIN1 and HSR203J) as well as a senescence marker (SAG12) are coordinately induced during these processes. Our result indicates that although there is evidence of some cross-talk between both cell death pathways, spatial and temporal characteristics of activation for these markers during hypersensitive response and senescence are distinct. These observations indicate that these markers are relatively specific for different cell death programs. Interestingly, they also revealed that a senescence-like process seems to be triggered at the periphery of the HR necrotic lesion. This suggests that cells committed to die during the HR might release a signal able to induce senescence in the neighboring cells. This phenomenon could correspond to the establishment of a second barrier against pathogens. Lastly, we used those cell death markers to better characterize cell death induced by copper and we showed that this abiotic induced cell death presents similarities with HR cell death.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1243-1255 |
| Number of pages | 13 |
| Journal | Plant Molecular Biology |
| Volume | 39 |
| Issue number | 6 |
| DOIs | |
| State | Published - Apr 1 1999 |
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All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Genetics
- Plant Science
Keywords
- Hypersensitive response
- Programmed cell death
- Senescence
- Tobacco mosaic virus
Cite this
}
Markers for hypersensitive response and senescence show distinct patterns of expression. / Pontier, Dominique; Gan, Susheng; Amasino, Richard M.; Roby, Dominique; Lam, Eric.
In: Plant Molecular Biology, Vol. 39, No. 6, 01.04.1999, p. 1243-1255.Research output: Contribution to journal › Article
TY - JOUR
T1 - Markers for hypersensitive response and senescence show distinct patterns of expression
AU - Pontier, Dominique
AU - Gan, Susheng
AU - Amasino, Richard M.
AU - Roby, Dominique
AU - Lam, Eric
PY - 1999/4/1
Y1 - 1999/4/1
N2 - Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily observed. Although several biochemical and morphological parameters have been described for various types of cell death in plants, the relationships existing between those different types of PCD events remain unclear. In this work, we set out to examine if two early molecular markers of HR cell death (HIN1 and HSR203J) as well as a senescence marker (SAG12) are coordinately induced during these processes. Our result indicates that although there is evidence of some cross-talk between both cell death pathways, spatial and temporal characteristics of activation for these markers during hypersensitive response and senescence are distinct. These observations indicate that these markers are relatively specific for different cell death programs. Interestingly, they also revealed that a senescence-like process seems to be triggered at the periphery of the HR necrotic lesion. This suggests that cells committed to die during the HR might release a signal able to induce senescence in the neighboring cells. This phenomenon could correspond to the establishment of a second barrier against pathogens. Lastly, we used those cell death markers to better characterize cell death induced by copper and we showed that this abiotic induced cell death presents similarities with HR cell death.
AB - Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily observed. Although several biochemical and morphological parameters have been described for various types of cell death in plants, the relationships existing between those different types of PCD events remain unclear. In this work, we set out to examine if two early molecular markers of HR cell death (HIN1 and HSR203J) as well as a senescence marker (SAG12) are coordinately induced during these processes. Our result indicates that although there is evidence of some cross-talk between both cell death pathways, spatial and temporal characteristics of activation for these markers during hypersensitive response and senescence are distinct. These observations indicate that these markers are relatively specific for different cell death programs. Interestingly, they also revealed that a senescence-like process seems to be triggered at the periphery of the HR necrotic lesion. This suggests that cells committed to die during the HR might release a signal able to induce senescence in the neighboring cells. This phenomenon could correspond to the establishment of a second barrier against pathogens. Lastly, we used those cell death markers to better characterize cell death induced by copper and we showed that this abiotic induced cell death presents similarities with HR cell death.
KW - Hypersensitive response
KW - Programmed cell death
KW - Senescence
KW - Tobacco mosaic virus
UR - http://www.scopus.com/inward/record.url?scp=0033121063&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033121063&partnerID=8YFLogxK
U2 - 10.1023/A:1006133311402
DO - 10.1023/A:1006133311402
M3 - Article
C2 - 10380810
AN - SCOPUS:0033121063
VL - 39
SP - 1243
EP - 1255
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 6
ER -