Mass spectra of permethyl derivatives of glyco-sphingolipids

R. W. Ledeen, S. K. Kundu, H. C. Price, J. W. Fong

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Mass spectra of the permethyl derivatives of a series of glycosphingolipids have been recorded. Fragments containing one, two and in some cases three sugar moieties were detected for the polyglycosyl compounds, several of these being of high intensity. The utility of such ions in establishing the position of hexosamine in an oligosaccharide chain was demonstrated. Low molecular weight gangliosides gave peaks corresponding to permethylated sialic acid under certain experimental conditions, permitting identification of N-acetyl- and N-glycoclylneuraminic acids. Glass sample containers appeared to catalyze destruction of these units. Peaks corresponding to the entire ceramide unit were detected but only in a few instances did these reflect the true ceramide composition. Specific fragments derived from the fatty acids and long-chain bases, respectively, were shown to be useful for qualitative identification of these components.

Original languageEnglish (US)
Pages (from-to)429-446
Number of pages18
JournalChemistry and Physics of Lipids
Issue number4
StatePublished - Dec 1974
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

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