Mast cell activation accelerates the normal rate of angiogenesis in the chick chorioallantoic membrane

Victor Rizzo, David O. DeFouw

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Using shell-less cultures of chick embryos, the effect of mast cell degranulation on normal rates of angiogenesis in the chorioallantoic membrane (CAM) was recorded from Day 6 to Day 9. Based on the centripetal ordering method with capillaries as the initial point of reference, the first two orders of pre- and postcapillary microvessels were evaluated. After infusion of compound 48/80 into the allantoic cavity, the normal proliferation rate of order-1 pre- and postcapillaries was markedly increased. Increases of second-order vessel numerical densities (number/cm2) were less than those of the order-1 vessels. Thus, length densities (mean length x numerical density) were increased for the order-1 vessels only. During the accelerated rate of CAM angiogenesis, endothelial restriction of FITC-dextran 150 remained uniformly high. Hence, secreted mast cell products likely worked synergistically with endogenous CAM angiogenic factors between Days 6 and 9, without increasing rates of macromolecular extravasation. However, histamine injection into the allantoic cavity at Day 10, in conjunction with calcium supplementation of the shell-less cultures at Day 9, elevated the rate of LITC-dextran 150 extravasation. Whether differentiation of this transendothelial pathway is required for enhancement of CAM angiogenesis after Day 10 remains uncertain.

Original languageEnglish (US)
Pages (from-to)245-257
Number of pages13
JournalMicrovascular Research
Volume52
Issue number3
DOIs
StatePublished - Nov 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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