Matrix assembly, cell polarization, and cell survival: analysis of peri-implantation development with cultured embryonic stem cells.

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A variety of mutations, including those affecting laminin expression and basement membrane, cause early embryonic lethality in the peri-implantation period. However, low cell numbers and inaccessibility of these small embryos make it difficult to study the molecular mechanisms that underlie these defects. Embryoid bodies cultured as suspended spherical cell aggregates derived from normal and defective embryonic stem cells provide a tractable experimental system with which the early developmental processes can be recapitulated under defined conditions. Thus, endoderm formation and maturation, basement membrane assembly and its signaling consequences, epiblast polarization, apoptosis, and cavitation can be studied using a combination of genetic, biochemical, cell, and molecular biology approaches.

Original languageEnglish (US)
Pages (from-to)113-125
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume329
StatePublished - 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Matrix assembly, cell polarization, and cell survival: analysis of peri-implantation development with cultured embryonic stem cells.'. Together they form a unique fingerprint.

Cite this