Measurement of telomere DNA content by dot blot analysis

Masayuki Kimura, Abraham Aviv

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Telomeres play a central role in human cancer, cardiovascular aging and possibly longevity. However, present methods to measure telomere length are fraught with shortcomings that limit their use. Here, we describe a novel method to measure the relative telomere DNA content by dot blot analysis. In each dot, the DNA content is measured by a DNA stain (Dx) and the telomeric DNA content is measured with a telomeric probe (T). The T normalized for Dx (T/Dx) of each dot is a measure of telomere content. The method requires ∼20ng of DNA per assay. Moreover, the T/Dx data are highly correlated linearly with mean telomere lengths derived from Southern blots of the terminal restriction fragments (r>0.96, P<0.0001). The method is also simple to use, has a relatively low interassay coefficient of variation (<6), retains its precision in moderately degraded DNA and can be forged for high throughput analysis. The method might help researchers and clinicians alike in understanding risks for and extent of human diseases.

Original languageEnglish (US)
Pages (from-to)e84
JournalNucleic acids research
Issue number12
StatePublished - Jul 2011

All Science Journal Classification (ASJC) codes

  • Genetics


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